您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and comparison of candidate odorant receptor genes in the olfactory and non-olfactory organs of Holotrichia oblita Faldermann by transcriptome analysis

文献类型： 外文期刊

作者： Li, Kebin 1 ; Wei, Hongshuang 1 ; Shu, Changlong 1 ; Zhang, Shuai 1 ; Cao, Yazhong 1 ; Luo, Chen 2 ; Yin, Jiao 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： Holotrichia oblita;Transcriptome;Odorant receptors;Ionotropic receptors;Tissue expression;Olfactory perception

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.674； 五年影响因子：2.941 ）

ISSN： 1744-117X

年卷期： 2017 年 24 卷

页码：

收录情况： SCI

摘要： A sophisticated olfactory system is part of the explanation for the prominence of insects among animals because of the essential roles of the olfactory system in foraging, host seeking, mating, ovipositing and avoiding toxic substances. In this study, we sequenced and analysed the transcriptomes of olfactory tissue (antennae) and non olfactory tissue (legs) of the scarab beetle, Holotrichia oblita Faldermann, which is a serious underground pest in China. We obtained approximately 80.2 million 150 bp reads that were assembled into 61,038 unigenes with an average length of 890 bp. Among the transcripts, 70% of the unigenes were annotated. A total of 44 odorant receptors (ORs) and 9 ionotropic receptors (IRs) were identified based on homology searches. Then, quantitative real-time PCR experiments were performed to investigate the expression patterns of 32 putative chemosensory genes. The results showed that these genes were highly expressed in olfactory organs (antennae) and might play a key role in the olfaction-related behaviours in H. oblita. Based on the results of our phylogenetic analysis and the detailed tissue and sex-biased expression characteristics, the different roles of the receptor proteins in the olfactory system were also indicated. The results of this study will provide the foundation for further understanding of the olfactory odorant receptors of H. oblita at the molecular level and ultimately help to develop novel targets for manipulating this pest.

  • 相关文献

[1]Development of Polymorphic Genic SSR Markers by Transcriptome Sequencing in the Welsh Onion (Allium fistulosum L.). Yang, Liuyi,Wen, Changlong,Zhao, Hong,Liu, Qianchun,Yang, Jingjing,Wang, Yongqin,Yang, Liuyi,Liu, Lecheng. 2015

[2]Transcriptome Analysis of Pepper (Capsicum annuum) Revealed a Role of 24-Epibrassinolide in Response to Chilling. Li, Jie,Yu, Jihua,Lyu, Jian,Zhang, Guobin,Feng, Zhi,Xie, Jianming,Yang, Ping,Kang, Jungen,Gan, Yantai,Gan, Yantai,Calderon-Urrea, Alejandro. 2016

[3]Transcriptome analysis and identification of induced genes in the response of Harmonia axyridis to cold hardiness. Tang, Bin,Liu, Xiao-Jun,Shi, Zuo-Kun,Shen, Qi-Da,Xu, Yan-Xia,Wang, Shi-Gui,Wang, Su,Zhang, Fan.

[4]Identification and sex expression profiling of odorant-binding protein genes in Trichogramma japonicum (Hymenoptera: Trichogrammatidae) using RNA-Seq. Wu, Jia-Dong,Shen, Zhao-Can,Hua, Hai-Qing,Li, Yuan-Xi,Zhang, Fan,Li, Yuan-Xi.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台