文献类型： 外文期刊

作者： Ma, Yun ¹ ; Chen, Ningbo ¹ ; Li, Rongrong ¹ ; Xu, Yongjie ¹ ; Li, Fen ¹ ; Li, Junya ² ; Gao, Shuxin ³ ; Li, Xiaofeng ⁴ ; Shi ¹ ;

作者机构： 1.Xinyang Normal Univ, Coll Life Sci, Xinyang 464000, Henan, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China

3.Inner Mongolia Univ Nationalities, Coll Anim Sci & Technol, Hohhot 028000, Inner Mongolia, Peoples R China

4.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Hubei, Peoples R China

5.Anim Husb Bur Ping Dingshan, Dingshan 467000, Henan, Peoples R China

关键词： Association analysis;Chinese indigenous cattle;LXR alpha gene;mRNA expression;Polymorphisms

期刊名称：JOURNAL OF APPLIED GENETICS （ 影响因子：3.24； 五年影响因子：2.756 ）

ISSN： 1234-1983

年卷期： 2014 年 55 卷 1 期

页码：

收录情况： SCI

摘要： Liver X receptor alpha (LXR alpha) has emerged as an important regulator of lipid and energy metabolism. In this study, to better understand the effects of LXR alpha gene on growth traits in cattle, the mRNA tissue expression patterns and the polymorphisms of some exons of LXR alpha were revealed. The expression profile of the bovine LXR alpha gene was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in 11 different Jiaxian cattle tissues and was found mainly expressed in spleen, liver, fat tissue, kidney, muscle, and lung. Meanwhile, it showed that four single nucleotide polymorphisms (SNPs), named g.1028 T > C, g.1514 T > C, g.2929G > A, and g.3493 T > C, were detected and 12 different haplotypes were constructed. Haplotype with CCGT was dominant with frequency of 40.8 %. There was a strong link between g.1028 T > C and g.1514 T > C (r (2) = 0.374). Association analysis of SNPs with growth- and body-related traits was carried out in 445 Chinese native cattle. The results displayed that the heterozygous genotypes of g.1028 T > C and g.1514 T > C showed a molecular heterosis on four performance traits related to body size: height at withers, body length, hipbone width, and hip width (P < 0.05). The multiple effects of four sites showed that the height at withers, body length, hipbone width, and hip width of individuals of TC-TC-GG-TT combined genotypes were significantly higher than other genotypes (P < 0.05). The effects of the four loci genotype combination on conformation traits were consistent with the effects of g.1028 T > C and g.1514 T > C loci. The SNPs of g.1028 T > C and g.1514 T > C of the bovine LXR alpha gene could be potential genetic markers for growth traits in cattle. These results suggest that LXR alpha gene is expressed in many tissues and may provide primary molecular information for further studies on body size traits in Chinese indigenous cattle.