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Artificially Increasing Cortical Tension Improves Mouse Oocytes Development by Attenuating Meiotic Defects During Vitrification

文献类型： 外文期刊

作者： Du, Xingzhu 1 ; Li, Jun 2 ; Zhuan, Qingrui 1 ; Zhang, Luyao 3 ; Meng, Lin 3 ; Ren, Panyu 3 ; Huang, Xiaohan 3 ; Bai, Jiachen 4 ; Wan, Pengcheng 5 ; Sun, Wenquan 4 ; Hou, Yunpeng 3 ; Zhu, Shien 1 ; Fu, Xiangwei 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod,Beijing Key, Minist Agr & Rural Affairs,Natl Engn Lab Anim Bre, Beijing, Peoples R China

2.Hebei Med Univ, Hosp 1, Dept Reprod Med, Reprod Med Ctr, Shijiazhuang, Hebei, Peoples R China

3.China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing, Peoples R China

4.Univ Shanghai Sci & Technol, Inst Biothermal Sci & Technol, Sch Med Instrument & Food Engn, Shanghai, Peoples R China

5.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, State Key Lab Sheep Genet Improvement & Hlth Bree, Shihezi, Peoples R China

关键词： oocyte vitrificaion; cortical tension; meiosis; spindle assembly checkpoint; aneuploidy

期刊名称：FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY （ 影响因子：6.081； 五年影响因子：6.576 ）

ISSN： 2296-634X

年卷期： 2022 年 10 卷

页码：

收录情况： SCI

摘要： Oocyte cryopreservation demonstrates great benefits in the conservation of animal germplasm resources and assisted reproductive technology. However, vitrification causes damages in oocytes, which would lead to the decrease of oocyte quality, and embryonic development post fertilization. Cytoskeleton plays an important role in regulating cell shape, organelle migration, cell division and mechanical signal transduction. Cortical tension is a reflection of the physiological state and contractile ability of cortical cytoskeleton. Appropriate cortical tension is prerequesite for normal oocyte meiosis. In the present study, oocyte cortical tension was examined by evaluating the levels of cortical tension-related protein pERM (Phospho-Ezrin/Radixin/Moesin) and pMRLC (Phospho-Myosin Light Chain 2). We found that the cortical tension of vitrified oocytes was decreased. Increasing cortical tension of vitrified oocytes by adding 10 mu g/ml ConA during in vitro culture could significantly improve the polar body extrusion rate and embryo development. Furthermore, increasing the cortical tension could improve spindle positioning, maintain kinetochore-microtubule (KT-MT) attachment, strengthen spindle assembly checkpoint (SAC) activity, and reduce the aneuploidy rate in vitrified oocytes. In conclusion, vitrification induced a remarkable decrease in cortical tension, and increasing the cortical tension could rescue the meiosis defect and improve oocyte quality.

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