广东省农业科学院机构知识库

Molecular cloning and virus-induced gene silencing of MiASB in the southern root-knot nematode, Meloidogyne incognita

文献类型：外文期刊

作者： Huang, Yonghong ¹ ; Mei, Mei ² ; Mao, Zhenchuan ² ; Lv, Shun ⁴ ; Zhou, Jiankun ⁴ ; Chen, Shi ⁴ ; Xie, Bingyan ² ;

作者机构： 1.Guangdong Acad Agr Sci, Fruit Res Inst, Minist Agr, Key Lab Biol & Genet Resource Utilizat Fruit Tree, Guangzhou 510640, Guangdong, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

3.Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China

4.Dongguan Inst Banana & Vegatable Sci, Dongguan 523061, Guangdong, Peoples R China

关键词： Meloidogyne spp;Mitochondrial ATP synthase;Gall;Control;Resistance;RNA interference

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （影响因子：1.907；五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 2014 年 138 卷 1 期

页码：

收录情况： SCI

摘要： Root-knot nematodes (Meloidogyne spp.), cause serious damage to agricultural production worldwide. In this study, we designed special primers based on the predicted Mitochondrial ATP synthase b subunit gene (ASB) sequence to clone the same gene in M. incognita (MiASB). The identity between the cloned MiASB and the predicted MiASB was as high as 100 %. Using the tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) system, we delivered MiASB RNAi triggers to the M. incognita feeding site on tomato seedlings, resulting in significantly fewer galls on the seedlings. Sixty days after inoculation with M. incognita, the number of root galls induced on the MiASB silence-treated seedlings was reduced by 64.3 % compared to that on the control seedlings, and reduced by 64.1 % compared to that on untreated control seedlings. This study revealed the MiASB silencing had a positive effect on the control of root-knot nematodes, and MiASB may be associated with the formation of galls caused by the nematode.

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意见箱

Molecular cloning and virus-induced gene silencing of MiASB in the southern root-knot nematode, Meloidogyne incognita

作者其他论文 更多>>

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RNA Sequencing Reveals that Endoplasmic Reticulum Stress and Disruption of Membrane Integrity Underlie Dimethyl Trisulfide Toxicity against Fusarium oxysporum f. sp cubense Tropical Race 4

The advancement of research on litchi and longan germplasm resources in China

Development of a high-efficiency gene knockout system for Pochonia chlamydosporia

Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic Fusarium oxysporum CS-20

意 见 箱

作者其他论文更多>>

意见箱