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A calcium-dependent protein kinase gene SpCPK33 from Solanum pennellii associated with increased cold tolerance in tomato

文献类型： 外文期刊

作者： Hu, Jiahui 1 ; Wang, Baike 2 ; Yang, Tao 2 ; Li, Ning 2 ; Yang, Haitao 2 ; Yu, Qinghui 1 ; Wang, Juan 2 ;

作者机构： 1.Xinjiang Agr Univ, Coll Hort, Urumqi, Xinjiang, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Hort Crops, Key Lab Hort Crop Genom Res & Genet Improvement Xi, Urumqi, Peoples R China

3.Xinjiang Acad Agr Sci, Hort Crops, Urumqi, Peoples R China

关键词： Solanum pennellii; SpCPK33; Cold stress; ROS

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：4.3； 五年影响因子：4.5 ）

ISSN： 0176-1617

年卷期： 2022 年 279 卷

页码：

收录情况： SCI

摘要： Calcium-dependent protein kinases (CDPKs, CPKs) represent a vital class of calcium sensors, which play a crucial role in plant growth, development and adaption to complex environmental stresses. Wild species tend to exhibit greater tolerance than cultivated species under environmental stress. Here, we isolated a calcium-dependent protein kinase gene SpCPK33 located primarily on the plasma membrane of abiotic-resistant species (Solanum pennellii LA0716). It was highly expressed in stems and leaves and was also induced by cold stress. Compared with WT plants, the overexpression of SpCPK33 in cultivated tomato (cv M82) enhanced its tolerance to cold stress. Transgenic lines demonstrated strong vitality under low temperature treatment. Moreover, the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were decreased in SpCPK33-overexpressing plants. The activities of antioxidant enzymes and the levels of osmotic regulatory substances were higher. The transcript levels of cold stress-related genes were up-regulated. In summary, the results indicate that SpCPK33-over-expressing transgenic plants experience less severe chilling injury under cold stress, and improved tomato cold tolerance by scavenging ROS accumulation and modulating the expression of stress-related genes.

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