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An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes

文献类型: 外文期刊

作者: Zhang, Zengyan 1 ; Liu, Xin 1 ; Wang, Xindong 1 ; Zhou, Miaoping; Zhou, Xianyao 1 ; Ye, Xingguo 1 ; Wei, Xuening 2 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Crop Sci,Agr Minist, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Beijing 100081, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci,Agr Minist, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol &

关键词: Bipolaris sorokiniana;common wheat;drought;MYB transcription factor;resistant response;Triticum aestivum

期刊名称:NEW PHYTOLOGIST ( 影响因子:10.151; 五年影响因子:10.475 )

ISSN: 0028-646X

年卷期: 2012 年 196 卷 4 期

页码:

收录情况: SCI

摘要: In this study, we report new insights into the function of a wheat (Triticum aestivum) MYB gene TaPIMP1 through overexpression and underexpression, and its underlying mechanism in wheat. Electrophoretic mobility shift and yeast-one-hybrid assays indicated that TaPIMP1 can bind to five MYB-binding sites including ACI, and activate the expression of the genes with the cis-element, confirming that TaPIMP1 is an MYB transcription activator. TaPIMP1-overexpressing transgenic wheat exhibited significantly enhanced resistance to the fungal pathogen Bipolaris sorokiniana and drought stresses, whereas TaPIMP1-underexpressing transgenic wheat showed more susceptibility to the stresses compared with untransformed wheat, revealing that TaPIMP1 positively modulates host-defense responses to B. sorokiniana and drought stresses. Microarray analysis showed that a subset of defense- and stress-related genes were up-regulated by TaPIMP1. These genes, including TaPIMP1, RD22, TLP4 and PR1a, were regulated by ABA and salicylic acid (SA). TaPIMP1-underexpressing transgenic wheat showed compromised induction of these stress-responsive genes following ABA and SA treatments. In summary, TaPIMP1, as a positive molecular linker, mediates resistance to B. sorokiniana and drought stresses by regulation of stress-related genes in ABA- and SA-signaling pathways in wheat. Furthermore, TaPIMP1 may provide a transgenic tool for engineering multiple-resistance wheat in breeding programs.

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