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Pathway redesign for deoxyviolacein biosynthesis in Citrobacter freundii and characterization of this pigment

文献类型： 外文期刊

作者： Jiang, Pei-xia 1 ; Wang, Hai-sheng 3 ; Xiao, Su 1 ; Fang, Ming-yue 1 ; Zhang, Rui-ping 1 ; He, Shu-ying 4 ; Lou, Kai 5 ;

作者机构： 1.Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, Beijing 100084, Peoples R China

2.Chinese Acad Sci, Dept Ind Microbiol & Biotechnol, Inst Microbiol, Beijing 100101, Peoples R China

3.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China

4.China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Jiangsu, Peoples R China

5.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Peoples R China

关键词： Violacein;Deoxyviolacein;Biosynthesis;Heterologous expression;Pathway redesign

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2012 年 94 卷 6 期

页码：

收录情况： SCI

摘要： Violacein (Vio) is an important purple pigment with many potential bioactivities. Deoxyviolacein, a structural analog of Vio, is always synthesized in low concentrations with Vio in wild-type bacteria. Due to deoxyviolacein's low production and difficulties in isolation and purification, little has been learned regarding its function and potential applications. This study was the first effort in developing a stable and efficient biosynthetic system for producing pure deoxyviolacein. A recombinant plasmid with vioabce genes was constructed by splicing using an overlapping extension-polymerase chain reaction, based on the Vio-synthesizing gene cluster of vioabcde, originating from Duganella sp. B2, and was introduced into Citrobacter freundii. With the viod gene disrupted in the Vio synthetic pathway, Vio production was completely abolished and the recombinant C. freundii synthesized only deoxyviolacein. Interestingly, vioe gene expression was strongly stimulated in the viod-deleted recombinant strain, indicating that viod disruptions could potentially induce polar effects upon the downstream vioe gene within this small operon. Deoxyviolacein production by this strain reached 1.9 g/L in shaker flasks. The product exhibited significant acid/alkali and UV resistance as well as significant inhibition of hepatocellular carcinoma cell proliferation at low concentrations of 0.1-1 mu M. These physical characteristics and antitumor activities of deoxyviolacein contribute to illuminating its potential applications.

  • 相关文献

[1]Reconstruction of the violacein biosynthetic pathway from Duganella sp B2 in different heterologous hosts. Jiang, Pei-xia,Zhang, Chong,Xing, Xin-Hui,Wang, Hai-sheng,Lou, Kai. 2010

[2]Optimization of culture conditions for violacein production by a new strain of Duganella sp B2. Jiang, Peixia,Lu, Yuan,Xing, Xin-Hui,Wang, Haisheng,Ruan, Zhiyong,Jiang, Ruibo,Lou, Kai,Wei, Dong. 2009

[3]Fed-batch fermentation of recombinant Citrobacter freundii with expression of a violacein-synthesizing gene cluster for efficient violacein production from glycerol. Yang, Cheng,Jiang, Peixia,Xiao, Su,Zhang, Chong,Xing, Xin-Hui,Lou, Kai.

[4]Production of violet pigment by a newly isolated psychrotrophic bacterium from a glacier in Xinjiang, China. Lu, Yuan,Wang, Liyan,Xue, Yuan,Zhang, Chong,Xing, Xin-Hui,Lou, Kai,Zhang, Zhidong,Li, Yong,Zhang, Guifeng,Bi, Jingxiu,Su, Zhiguo.

[5]High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Fang, Ming-Yue,Zhang, Chong,Xing, Xin-Hui,Yang, Song,Cui, Jin-Yu,Jiang, Pei-Xia,Lou, Kai,Wachi, Masaaki. 2015

[6]Association Analysis of the nced and rab28 Genes with Phenotypic Traits Under Water Stress in Maize. Su, Zhijun,Li, Xinhai,Hao, Zhuanfang,Xie, Chuanxiao,Li, Mingshun,Weng, Jianfeng,Zhang, Degui,Zhang, Shihuang,Su, Zhijun,Li, Xinhai,Hao, Zhuanfang,Xie, Chuanxiao,Li, Mingshun,Weng, Jianfeng,Zhang, Degui,Zhang, Shihuang,Liang, Xiaoling,Su, Zhijun,Wang, Zhigang,Gao, Julin.

[7]A high-throughput screening method for identifying lycopene-overproducing E. coli strain based on an antioxidant capacity assay. Xu, Xian,Jin, Weiyue,Xu, Qing,Li, Shuang,Huang, He,Jiang, Ling,Zhang, Zhidong,Huang, He.

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