Characterization and expression analysis of six MADS-box genes in maize (Zea mays L.)
文献类型: 外文期刊
作者: Zhang, Zhongbao 1 ; Li, Huiyong 1 ; Zhang, Dengfeng 1 ; Liu, Yinghui 1 ; Fu, Jing 1 ; Shi, Yunsu 1 ; Song, Yanchun 1 ; Wa 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
3.Hebei North Univ, Zhangjiakou 075000, Peoples R China
关键词: Abiotic stress;Expression;MADS-box;Maize;Transcriptional activity
期刊名称:JOURNAL OF PLANT PHYSIOLOGY ( 影响因子:3.549; 五年影响因子:4.164 )
ISSN: 0176-1617
年卷期: 2012 年 169 卷 8 期
页码:
收录情况: SCI
摘要: MADS-box genes encode a family of transcription factors, which control diverse developmental processes in flowering plants, with organs ranging from roots, flowers and fruits. In this study, six maize cDNAs encoding MADS-box proteins were isolated. BLASTX searches and phylogenetic analysis indicated that the six MADS-box genes belonging to the AGL2-like clade. qRT-PCR analysis revealed that these genes had differential expression patterns in different organs in maize. The results of yeast one-hybrid system indicated that the protein ZMM3-1, ZMM3-2, ZMM6, ZMM7-L, ZMM8-L and ZMM14-L had transcriptional activation activity. Subcellular localization of ZMM7-L demonstrated that the fluorescence of ZMM7-L-GFP was mainly detected in the nuclei of onion epidermal cells. qRT-PCR analysis for expression pattern of ZMM7-L showed that the gene was up-regulated by abiotic stresses and down-regulated by exogenous ABA. The germination rates of over-expression transgenic lines were lower than that of the wild type on medium with 150 mM NaCl, 350 mM mannitol. These results indicated that ZMM7-L might be a negative transcription factor responsive to abiotic stresses. (C) 2012 Elsevier GmbH. All rights reserved.
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