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Virus-like particles of hepatitis B virus core protein containing five mimotopes of infectious bursal disease virus (IBDV) protect chickens against IBDV

文献类型： 外文期刊

作者： Wang, Yong-shan 1 ; Ouyang, Wei 1 ; Liu, Xiao-juan 1 ; He, Kong-wang 1 ; Yu, Sheng-qing 3 ; Zhang, Hai-bin 2 ; Fan, Ho 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Vet Med, Dept Microbiol & Immunol, Nanjing 210095, Jiangsu, Peoples R China

3.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

关键词： Infectious bursal disease virus;Hepatitis B virus core particle;Virus-like particle;Chimeric VLP;Mimotope vaccine

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2012 年 30 卷 12 期

页码：

收录情况： SCI

摘要： Current infectious bursal disease virus (IBDV) vaccines suffer from maternal antibody interference and mimotope vaccines might be an alternative. Previously we demonstrated an IBDV VP2 five-mimotope polypeptide, 5EPIS, elicited protective immunity in chickens. In the current study, the 5epis gene was inserted into a plasmid carrying human hepatitis B virus core protein (HBc) gene at its major immunodominant region site. The recombinant gene was efficiently expressed in Escherichia coli to produce chimeric protein HBc-5EPIS which self-assembles to virus-like particles (VLP). Two-week old specific-pathogen-free chickens were immunized intramuscularly with HBc-5EPIS VLP or 5EPIS polypeptide without adjuvant (50 mu g/injection) on day 0, 7, 14 and 21. Anti-5EPIS antibody was first detected on day 7 and day 21 in HBc-5EPIS and 5EPIS groups, respectively; on day 28, anti-5EPIS titers reached 12,800 or 1600 by ELISA, and 3200 or 800 by virus neutralization assay in HBc-5EPIS and 5EPIS groups, respectively. No anti-5EPIS antibody was detected in the buffer control group throughout the experiment. Challenge on day 28 with a virulent IBDV strain (GX8/99) resulted in 100%, 40.0% and 26.7% survival for chickens immunized with HBc-5EPIS, 5EPIS and buffer, respectively. These data suggest epitope presentation on chimeric VLP is a promising approach for improving mimotope vaccines for IBDV. (C) 2012 Elsevier Ltd. All rights reserved.

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