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The F-box protein CPR1/CPR30 negatively regulates R protein SNC1 accumulation

文献类型： 外文期刊

作者： Gou, Mingyue 1 ; Shi, Zhenying 1 ; Zhu, Ying 1 ; Bao, Zhilong 1 ; Wang, Guoying 4 ; Hua, Jian 1 ;

作者机构： 1.Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA

2.Shanghai Inst Plant Physiol & Ecol, Shanghai 20032, Peoples R China

3.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

关键词： CPR30;CPR1;SNC1;F-box protein;R protein;protein degradation

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2012 年 69 卷 3 期

页码：

收录情况： SCI

摘要： Disease resistance (R) proteins, as central regulators of plant immunity, are tightly regulated for effective defense responses and to prevent constitutive defense activation under non-pathogenic conditions. Here we report the identification of an F-box protein CPR1/CPR30 as a negative regulator of an R protein SNC1 likely through SCF (Skp1-cullin-F-box) mediated protein degradation. The cpr1-2 (cpr30-1) loss-of-function mutant has constitutive defense responses, and it interacts synergistically with a gain-of function mutant snc1-1 and a bon1-1 mutant where SNC1 is upregulated. The loss of SNC1 function suppresses the mutant phenotypes of cpr1-2 and cpr1-2 bon1-1, while overexpression of CPR1 rescues mutant phenotypes of both bon1-1 and snc1-1. Furthermore, the amount of SNC1 protein is upregulated in the cpr1-2 mutant and down-regulated when CPR1 is overexpressed. The regulation of SNC1 by CPR1 is dependent on the 26S proteosome as a protease inhibitor MG132 stabilizes SNC1 and reverses the effect of CPR1 on SNC1. Interestingly, CPR1 is induced after infection of both virulent and avirulent pathogens similarly to the other negative defense regulator BON1. Thus, this study reveals a new mechanism in R protein regulation likely through protein degradation and suggests negative regulation as a critical component in fine control of plant immunity.

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