文献类型： 外文期刊

作者： Zhu, Huaguo ¹ ; Wang, Fuqiu ² ; Xu, Zhongping ² ; Wang, Guanying ² ; Hu, Lisong ³ ; Cheng, Junyong ⁴ ; Ge, Xianhong ² ; Liu, Jinxuan ² ; Chen, Wei ⁵ ; Li, Qiang ⁵ ; Xue, Fei ⁷ ; Liu, Feng ⁷ ; Li, Wenying ¹ ; Wu, Lan ¹ ; Cheng, Xinqi ¹ ; Tang, Xinxin ¹ ; Yang, Chaochen ¹ ; Lindsey, Keith ⁸ ; Zhang, Xianlong ² ; Ding, Fang ⁹ ; Hu, Haiyan ¹⁰ ; Hu, Xiaoming ¹ ; Jin, Shuangxia ² ;

作者机构： 1.Huanggang Normal Univ, Coll Biol & Agr Resources, Huanggang, Hubei, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan, Hubei, Peoples R China

3.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Hainan, Peoples R China

4.Hubei Acad Forestry, Wuhan, Hubei, Peoples R China

5.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China

6.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan, Hubei, Peoples R China

7.Shihezi Univ, Coll Agr, Shihezi, Xinjiang, Peoples R China

8.Univ Durham, Dept Biosci, Durham, England

9.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Key Lab Plant Pathol, Wuhan, Hubei, Peoples R China

10.Hainan Univ, Sanya Inst Breeding & Multiplicat, Sch Breeding & Multiplicat, Sanya, Hainan, Peoples R China

关键词： Oil-Camellia (Camellia oleifera); hexaploid; chromosome-scale genome assembly; whole-genome duplication; camellia oil biosynthesis

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.1； 五年影响因子：11.6 ）

ISSN： 1467-7644

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Oil-Camellia (Camellia oleifera), belonging to the Theaceae family Camellia, is an important woody edible oil tree species. The Camellia oil in its mature seed kernels, mainly consists of more than 90% unsaturated fatty acids, tea polyphenols, flavonoids, squalene and other active substances, which is one of the best quality edible vegetable oils in the world. However, genetic research and molecular breeding on oil-Camellia are challenging due to its complex genetic background. Here, we successfully report a chromosome-scale genome assembly for a hexaploid oil-Camellia cultivar Changlin40. This assembly contains 8.80 Gb genomic sequences with scaffold N50 of 180.0 Mb and 45 pseudochromosomes comprising 15 homologous groups with three members each, which contain 135 868 genes with an average length of 3936 bp. Referring to the diploid genome, intragenomic and intergenomic comparisons of synteny indicate homologous chromosomal similarity and changes. Moreover, comparative and evolutionary analyses reveal three rounds of whole-genome duplication (WGD) events, as well as the possible diversification of hexaploid Changlin40 with diploid occurred approximately 9.06 million years ago (MYA). Furthermore, through the combination of genomics, transcriptomics and metabolomics approaches, a complex regulatory network was constructed and allows to identify potential key structural genes (SAD, FAD2 and FAD3) and transcription factors (AP2 and C2H2) that regulate the metabolism of Camellia oil, especially for unsaturated fatty acids biosynthesis. Overall, the genomic resource generated from this study has great potential to accelerate the research for the molecular biology and genetic improvement of hexaploid oil-Camellia, as well as to understand polyploid genome evolution.