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Establishment and biological characteristics of Piedmontese cattle fibroblast line

文献类型： 外文期刊

作者： Wang, Hui 1 ; Liu, Di 2 ; Jin, Dapeng 1 ; Guo, Yu 1 ; Guan, Weijun 1 ; Ma, Yuehui 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

2.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

3.NE Forestry Univ, Coll Wildlife Resources, Harbin 150040, Peoples R China

关键词： Piedmontese cattle;fibroblast line;biological characterization

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 79 期

页码：

收录情况： SCI

摘要： A fibroblast line was successfully established from ear marginal tissues of Piedmontese cattle by direct culturing of explants. Biological analysis showed that the population doubling time (PDT) for reviving cells was approximately 24 h. The average viability of the cells was 96.6% before freezing and 92.7% after thawing. Isoenzyme polymorphorism of lactic dehydrogenase (LDH) and malic dehydrogenase (MDH) ruled out cross-contamination among cell lines. Karyotyping showed that the proportion of cells with chromosome number 2n = 60 was above 92%. Tests for bacteria, fungi, viruses and mycoplasmas were negative. The transfection efficiencies of pEGFP-N3, pEYFP-N1 and pDsRed1-N1 were between 7.1 and 30.8%; fluorescences were homogenously distributed throughout cytoplasm and nucleus except in some cryptomeric vesicles. Every index of the Piedmontese cattle cell line met the quality control standards of the American Type Culture Collection (ATCC).

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