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Arabidopsis E3 Ubiquitin Ligases PUB22 and PUB23 Negatively Regulate Drought Tolerance by Targeting ABA Receptor PYL9 for Degradation

文献类型： 外文期刊

作者： Zhao, Jinfeng 1 ; Zhao, Linlin 2 ; Zhang, Ming 1 ; Zafar, Syed Adeel 1 ; Fang, Jingjing 1 ; Li, Ming 3 ; Zhang, Wenhui 2 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

2.Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Peoples R China

3.Northeast Agr Univ, Coll Agr, Harbin 150030, Heilongjiang, Peoples R China

4.Heilongjiang Acad Agr Sci, Ind Crop Inst, Harbin 150086, Heilongjiang, Peoples R China

关键词： ABA receptor;cell-free degradation assay;degradation;drought tolerance;protein extract;U-box E3 Ubiquitin ligases

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2017 年 18 卷 9 期

页码：

收录情况： SCI

摘要： Drought causes osmotic stress and rapidly triggers abscisic acid (ABA) accumulation in plants. The roles of various ABA receptors in drought tolerance and molecular mechanisms regulating ABA receptor stability needs to be elucidated. Here, we report that Arabidopsis plants overexpressing PYL9, one of the 14 pyrabactin resistance (PYR)/pyrabactin resistance-like (PYL)/regulatory component of ABA receptors (RCAR) family ABA receptors, gained drought tolerance trait. Osmotic stress induced accumulation of the PYL9 protein, which was regulated by the 26S proteasome. PYL9 interacted with two highly homologous plant U-box E3 ubiquitin ligases PUB22 and PUB23. In the cell-free degradation assay, the degradation of GST-PYL9 was accelerated in protein extract from plants overexpressing PUB22 but slowed down in protein extract from the pub22 pub23 double mutant. The in vivo decay of Myc-PYL9 was significantly reduced in the pub22 pub23 double mutant as compared with the wild-type. Additionally, PUB22 also interacted with other ABA receptors such as PYL5, PYL7 and PYL8. Considering the improved drought tolerance in the pub22 pub23 double mutant in previous studies, our results suggest that PUB22 and PUB23 negatively regulate drought tolerance in part by facilitating ABA receptors degradation.

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[1]The Arabidopsis U-box E3 ubiquitin ligase PUB30 negatively regulates salt tolerance by facilitating BRI1 kinase inhibitor 1 (BKI1) degradation. Zhang, Ming,Li, Ming,Zhang, Ming,Zhao, Jinfeng,Patil, Suyash Bhimgonda,Fang, Jingjing,Li, Xueyong,Zhang, Ming,Li, Long,Yang, Yuhong,Zhao, Linlin,Zhang, Wenhui. 2017

[2]Yield performances of japonica introgression lines selected for drought tolerance in a BC breeding programme. He, Y. X.,Zheng, T. Q.,Wang, L. F.,Gao, Y. M.,Zhai, H. Q.,Xu, J. L.,Zhu, L. H.,Li, Z. K.,He, Y. X.,Xu, Z. J.,He, Y. X.,Hao, X. B.,Hua, Z. T.,Li, Z. K.. 2010

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