您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

CRISPR-Cas9-mediated editing of GmARM improves resistance to multiple stresses in soybean

文献类型： 外文期刊

作者： Luo, Tingting 1 ; Ma, Chongxuan 1 ; Fan, Yuanhang 1 ; Qiu, Zhendong 1 ; Li, Ming 2 ; Tian, Yusu 1 ; Shang, Yuzhuo 1 ; Liu, Chang 1 ; Cao, Qingqian 1 ; Peng, Yuhan 1 ; Zhang, Shuzhen 1 ; Liu, Shanshan 1 ; Song, Bo 1 ;

作者机构： 1.Northeast Agr Univ, Soybean Res Inst, Key Lab Soybean Biol, Chinese Educ Minist, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Keshan Branch, Qiqihar 161000, Peoples R China

3.Harbin Normal Univ, Coll Life Sci & Technol, Key Lab Mol & Cytogenet, Harbin 150025, Peoples R China

关键词： Soybean; Stress resistance; GmARM; CRISPR/Cas9

期刊名称：PLANT SCIENCE （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0168-9452

年卷期： 2024 年 346 卷

页码：

收录情况： SCI

摘要： The growth and development of soybean plants can be affected by both abiotic and biotic stressors, such as saline-alkali stress and Phytophthora root rot. In this study, we identified a stress-related gene-GmARM-whose promoter contained several hormone-response and stress-regulatory elements, including ABRE, TCA element, STRE, and MBS. qRT-PCR analysis showed that the expression of GmARM was the highest in seeds at 55 days after flowering. Furthermore, this gene was upregulated after exposure to saline-alkali stress and Phytophthora root rot infection at the seedling stage. Thus, we generated GmARM mutants using the CRISPR-Cas9 system to understand the role of this gene in stress response. T3 plants showed significantly improved salt tolerance, alkali resistance, and disease resistance, with a significantly higher survival rate than the wildtype plants. Moreover, mutations in GmARM affected the expression of related stress-resistance genes, indicating that GmARM mutants achieved multiple stress tolerance. Therefore, this study provides a foundation for further exploration of the genes involved in resistance to multiple stresses in soybean that can be used for breeding multiple stressresistance soybean varieties.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台