文献类型： 外文期刊

作者： Dai, Hongsheng ¹ ; Lv, Chunyi ³ ; Huang, Zhiwei ² ; Shen, Yuhu ¹ ; Shi, Ping ¹ ;

作者机构： 1.Qinghai Univ, Acad Agr & Forestry Sci, Qinghai Acad Agr & Forestry Sci, Lab Res & Utilizat Qinghai Tibet Plateau Germplasm, Xining 810016, Peoples R China

2.Donghua Univ, Coll Biol Sci & Med Engn, Shanghai, Peoples R China

3.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Qinghai Prov Key Lab Crop Mol Breeding,Innovat Aca, Xining, Peoples R China

关键词： Cadmium; yeast; glutathione redox; YCF1; bioremediation; detoxification

期刊名称：TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY （ 影响因子：1.1； 五年影响因子：1.4 ）

ISSN： 0277-2248

年卷期： 2024 年 106 卷 1-10 期

页码：

收录情况： SCI

摘要： Divalent cadmium is a toxic environmental pollutant to biological systems. This study confirmed that it causes oxidative stress in yeast cells. The total glutathione levels in yeast cells significantly decreased with increasing cadmium concentrations. The glutathione precursor cysteine induced a concentration-dependent reduction in the superoxide production and malondialdehyde content. The glutathione synthesis deficient strains gsh1 Delta, gsh2 Delta, and cysteine synthesis deficient strain (cys3 Delta) were more sensitive to cadmium than the wild type BY4741, and glutathione could increase the resistance of gsh1 Delta, gsh2 Delta, and cys3 Delta to cadmium, while it could not reverse the sensitivity of ycf1 Delta to cadmium. In addition, the overexpression of ERO1 could enhance the sensitivity of the wild-type and gsh1 Delta cells to cadmium, indicating that the detoxification of cadmium depends on the intracellular glutathione redox levels. However, the prerequisite is that the chelates formed by glutathione and cadmium (GS Cd SG) can be transported to the vacuole by YCF1. Therefore, the reduced glutathione helps to detoxify cadmium in a YCF1-dependent manner in yeast cells. This study contributes to understanding how yeast detoxifies cadmium, which will allow the development of yeast cells for bioremediation of cadmium pollution.