文献类型： 外文期刊

作者： Huang, Zhiwei ¹ ; Xiao, Fulong ¹ ; Wang, Qiao ¹ ; Zhang, Xiaojuan ¹ ; Shen, Yuhu ² ; Deng, Yunxia ¹ ; Shi, Ping ³ ;

作者机构： 1.Donghua Univ, Coll Biol Sci & Med Engn, 2999 Renmin Rd, Shanghai 201620, Peoples R China

2.Chinese Acad Sci, Northwest Inst Plateau Biol, Innovat Acad Seed Design, Key Lab Adaptat & Evolut Plateau Biota,Qinghai Pro, Xining 810008, Peoples R China

3.Qinghai Univ, Acad Agr & Forestry Sci, Qinghai Acad Agr & Forestry Sci, Lab Res & Utilizat Qinghai Tibet Plateau Germplasm, Xining 810016, Peoples R China

4.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

关键词： BSC2; Saccharomyces cerevisiae; AmB resistance; Sodium/potassium balance; FLO pathway

期刊名称：RESEARCH IN MICROBIOLOGY （ 影响因子：3.4； 五年影响因子：3.7 ）

ISSN： 0923-2508

年卷期： 2024 年 175 卷 8 期

页码：

收录情况： SCI

摘要： Previous studies on BSC2 have shown that it enhances yeast cell resistance to AmB via antioxidation and induces multidrug resistance by contributing to biofilm formation. Herein, we found that BSC2 over- expression could reverse the sensitivity of pmp30 to AmB and help the tested strains restore the intracellular sodium/potassium balance under exposure to AmB. Meanwhile, overexpression of the chitin gene CHS2 could simulate BSC2 to reverse the sensitivity of pmp30 and nha10 to high salt or AmB. However, BSC2 overexpression in flo110 failed to induce AmB resistance, form biofilms, and affect cell wall biogenesis, while CHS2 overexpression compensated the resistance of flo110 to AmB. Additionally, BSC2 levels were positively correlated with maintaining cell membrane integrity under exposure to AmB, CAS, or a combination of both. BSC2 overexpression in nha10 exhibited a similar function of CHS2, which can compensate for the sensitivity of the mutant to high salt. Altogether, the results demonstrate for the first time that BSC2 may promote ion equilibrium by strengthening cell walls and inhibiting membrane damage in a FLO path-dependent manner, thus enhancing the resistance of yeast cells to AmB. This study also reveals the possible mechanism of antifungal drugs CAS and AmB combined to inhibit fungi. (c) 2024 Institut Pasteur. Published by Elsevier Masson SAS. All rights are reserved, including those for text and data mining, AI training, and similar technologies.