Identification and molecular characterization of two liver expressed antimicrobial peptides (LEAPs) in Amur grayling (Thymallus arcticus grubei Dybowski, 1869)
文献类型： 外文期刊
作者： Cao, Baorui 1 ; Liu, Enhui 1 ; Huang, Tianqing 1 ; Gu, Wei 1 ; Wang, Gaochao 1 ; Wang, Bingqian 1 ; Xu, Gefeng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Key Lab Freshwater Aquat Biotechnol & Breeding, Minist Agr & Rural Affairs, Harbin, Peoples R China
2.Heilongjiang River Fisheries Res Inst, HeSong St 232, Harbin 150070, Peoples R China
关键词： Thymallus arcticus grubei Dybowski; LEAP-1; LEAP-2; Antimicrobial peptides; Antibacterial immunity
期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）
ISSN： 0044-8486
年卷期： 2024 年 581 卷
页码：
收录情况： SCI
摘要： Antimicrobial peptides (AMPs) are part of the innate immune response against bacteria. Liver expressed antimicrobial peptides (LEAPs) are one of the most important antimicrobial peptides in fish and belong to a family of cysteine-rich antimicrobial peptides with antimicrobial activity against a variety of pathogens. In this study, we cloned and identified two liver expressed antimicrobial peptides (TaLEAP-1, TaLEAP-2) from Thymallus arcticus grubei, functional studies were performed and the two synthesized TaLEAPs peptides were examined in vitro bacterial inhibition. The results showed that the open reading frame lengths of TaLEAP-1 and TaLEAP-2 were 267 bp and 258 bp, respectively. TaLEAP-1 is a beta-sheet polypeptide containing 89 amino acids, and TaLEAP-2 is an alpha-helix with beta-sheet polypeptide containing 85 amino acids. Tissue distribution showed that both TaLEAPs were widely expressed in ten tissues of T. arcticus grubei, with the highest expression in the liver. During the bacterial challenge with Aeromonas salmonicida and Aeromonas hydrophila, two LEAPs genes were differentially expressed. TaLEAP-1 has broad-spectrum antibacterial activity against a variety of bacteria. TaLEAP-1 damages bacteria by disrupting cell membranes, showing that TaLEAP-1 has potent antibacterial effects. Cytotoxicity assay results showed that TaLEAP-1 was not toxic and TaLEAP-2 was slightly toxic to RTG-2. The results of this study provide strategies for resistance to bacterial and fungal infections in T. arcticus grubei.
- 相关文献
作者其他论文 更多>>
-
Comparative metabolomics analysis based on LC-MS method reveals regulatory difference in lipid metabolism and fatty acids metabolism between diploid and triploid rainbow trout (Oncorhynchus mykiss)
作者：Li, Chenhui;Chen, Zhongxiang;Qin, Dongli;Xu, Gefeng;Gao, Lei;Hao, Qirui;Du, Ningning;Fan, Ze;Sun, Yanchun;Wang, Peng;Li, Chenhui;Wang, Peng;Li, Chenhui;Wang, Peng;Li, Chenhui;Wang, Peng
关键词：Metabolites; Polyunsaturated fatty acid (PUFA); Triploid rainbow trout; Diploid rainbow trout
-
Development of polymorphic simple sequences repeats markers from whole gene resequencing data comparison of 68 Oncorhynchus mykiss
作者：Ma, Haibing;Xu, Gefeng;Ma, Haibing;Huang, Tianqing;Liu, Enhui;Wang, Gaochao;Gu, Wei;Xu, Gefeng;Xu, Gefeng
关键词：rainbow trout (Oncorhynchus mykiss); salmon; population genetics; microsatellite markers; bioinformatics
-
A chromosome-level genome assembly and evolutionary analysis of Coregonus ussuriensis Berg
作者：Huang, Tianqing;Liu, Enhui;Cao, Baorui;Li, Wenwen;Wang, Gaochao;Gu, Wei;Ma, Haibing;Wang, Bingqian;Xu, Gefeng;Dong, Fulin
关键词：
-
Enhancing Growth and Intestinal Health in Triploid Rainbow Trout Fed a Low-Fish-Meal Diet through Supplementation with Clostridium butyricum
作者：Wang, Chang'an;Li, Fangyuan;Wang, Di;Lu, Shaoxia;Han, Shicheng;Gu, Wei;Liu, Hongbai;Wang, Chang'an;Jiang, Haibo;Li, Zhuang
关键词：triploid rainbow trout; C. butyricum; low-fish-meal diet; growth performance; intestinal health
-
Protective effects of polysaccharide from Atractylodes macrocephala Koidze (PAMK) as a feed additive on growth performance, immunity, antioxidant capacity, and disease resistance against Aeromonas salmonicida in rainbow trout (Oncorhynchus mykiss)
作者：Lu, Shaoxia;Wang, Chang'an;Wang, Di;Shi, Honghe;Wang, Shidi;Xu, Gefeng;Liu, Hongbai;Han, Shicheng;Lu, Shaoxia;Wang, Di;Shi, Honghe;Wang, Shidi;Liu, Hongbai;Han, Shicheng;Bian, Xunwen
关键词：Polysaccharide from Atractylodes macrocephala koidze (PAMK); Antioxidant capacity; Immune response; Oncorhynchus mykiss
-
Influence of increasing acclimation temperature on growth, digestion, antioxidant capacity, liver transcriptome and intestinal microflora of Ussruri whitefish Coregonus ussuriensis Berg
作者：Ge, Kaibo;Fan, Ze;Huang, Tianqing;Gu, Wei;Wang, Gaochao;Liu, Enhui;Pan, Runlei;Li, Datian;Sun, Yunchao;Yao, Zuochun;Wang, Liwei;Zhao, Cheng;Xu, Gefeng;Ge, Kaibo;Huang, Tianqing;Gu, Wei;Wang, Gaochao;Liu, Enhui;Pan, Runlei;Li, Datian;Sun, Yunchao;Yao, Zuochun;Xu, Gefeng;Fan, Ze
关键词：Ussruri whitefish Coregonus ussuriensis Berg; Temperature; Growth performance; Liver transcriptional responses; Intestinal microflora
-
Genome-wide identification of simple sequence repeats and development of polymorphic SSR markers in brown trout (Salmo trutta)
作者：Ma, Haibing;Huang, Tianqing;Gu, Wei;Liu, Enhui;Wang, Gaochao;Wang, Bingqian;Xu, Gefeng;Zhou, Jianshe
关键词：Brown trout (Salmo trutta); Microsatellite markers; Population genetics; Bioinformatics; Salmon; Genetic resource conservation
