文献类型： 外文期刊

作者： Zhao, Junliang ¹ ; Liang, Aihua ² ; Zhu, Zhen ³ ; Tang, Yixiong ⁴ ;

作者机构： 1.Shanxi Acad Agr Sci, Inst Vegetables, Taiyuan 030031, Peoples R China

2.Shanxi Univ, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030009, Peoples R China

3.Chinese Acad Sci, Genet Inst, Beijing 100101, Peoples R China

4.Chinese Acad Agr Sci, Ctr Biotechnol, Beijing 100081, Peoples R China

关键词： Agrobacterium tumefaciens;anti-bacterial peptide;Chinese cabbage;cowpea trypsin inhibitor;transformation

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2006 年 150 卷 3 期

页码：

收录情况： SCI

摘要： An efficient transformation system for Chinese cabbage cotyledon explants was developed using Agrobacterium tumefaciens strains LBA4404 harbouring the plasmid pMOG411 and the plasmid pBin Omega SCK respectively. Various factors affecting the transformation efficiency and subsequent regeneration were identified. The age of seedlings, growth conditions and status of Agrobacterium suspension, preculture of explants, cocultivation time, ratio between Agrobacterium and explants, acetosyringone and concentration of kanamycin had a significant influence on transformation frequency and plant regeneration. The presences of the antibacterial peptide gene and the cowpea trypsin inhibitor gene in those selected shoots in kanamycin medium were confirmed by PCR, Southern blotting and Northern blotting, The frequency of regeneration from cotyledons was analyzed in eight parental lines of Chinese cabbage and five lines were suitable for obtaining transformed plants, among which Qingmaye C and Qingmaye D were more responsive than other lines.