Development and identification of wheat-Haynaldia villosa T6DL.6VS chromosome translocation lines conferring resistance to powdery mildew
文献类型： 外文期刊
作者： Li, H 1 ; Chen, X 2 ; Xin, ZY 2 ; Ma, YZ 3 ; Xu, HJ; Chen, XY; Jia, X;
作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Crop Genet & Breeding, Beijing 100081, Peoples R China
2.Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Crop Genet & Breeding, Beijing 100081, Peoples R China; Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China; Hebei Acad Agr & Forestry Sci, Inst Food & Oil Crops, Key Lab Crop Genet & Breeding Hebei Prov, Shijiazhuang 050031, Hebei Province, Peoples R China
3.Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Crop Genet & Breeding, Beijing 100081, Peoples R China; Chinese Acad Sci, Inst Genet & Dev B
关键词： Triticum durum;Haynaldia villosa;biochemical analysis;cytogenetic analysis;genomic in situ hybridization;powdery mildew resistance;RFLP;T6DL.6VS translocation lines
期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）
ISSN： 0179-9541
年卷期： 2005 年 124 卷 2 期
页码：
收录情况： SCI
摘要： Three lines conferring resistance to powdery mildew, Pm97033, Pm97034 and Pm97035, were developed from the cross of Triticum durum-Haynaldia villosa amphidiploid TH3 and wheat cv. 'Wan7107' via backcrosses, immature embryo and anther culture. Genomic in situ hybridization analysis showed that these lines were disomic translocation lines. Cytogenetic analysis indicated that the F-1 plants of crosses between the three translocation lines and 'Wan7107' and crosses between the three translocation lines and substitution line 6V(6D) formed 21 bivalents at meiotic metaphase I. Aneuploid analysis with 'Chinese Spring' double ditelocentric stocks indicated that the translocated chromosomes were related to chromosome 6D. Biochemical and restriction fragment-length polymorphism (RFLP) analyses showed that the translocation lines lacked a specific band of 6VL of H. villosa compared with the substitution and addition lines but possessed specific markers on the short arm of the 6V chromosome of H. villosa. The three translocation lines lacked specific biochemical loci and RFLP markers located on chromosome 6DS. The results confirmed that Pm97033, Pm97034 and Pm97035 were T6DL.6VS translocation lines.
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