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Molecular cytogenetic identification of Triticum aestivum Secale cereale substitution and addition lines

文献类型： 外文期刊

作者： Li, HJ 1 ; Zhu, ZQ 2 ; Zhang, YM 2 ; Guo, BH 2 ; Wen, YX 2 ; Jia, X 2 ;

作者机构： 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China

2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China; Hebei Acad Agr & Forestry Sci, Inst Food & Oil Crops, Shijiazhuang 050031, Peoples R China; Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China

关键词： Triticum aestivum;triticale;Secale cereale;tissue culture;substitution line;addition line;fluorescence in situ hybridization

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 1998 年 40 卷 1 期

页码：

收录情况： SCI

摘要： Two substitution lines, designated as 930498 and 930483, and one addition line, designated as 930029, via F-o immature embryo culture of Triticum aestivum x octoploid triticale (x Triticosecale Wittmack) were identified. Fluorescence in situ hybridization (FISH) using total genomic DNA of rye (Secale cereale L.) as probe corroborated the existence of rye chromosomes, further confirmed through chromosome paring at meiotic metaphase I, C-banding and glutenin SDS-PAGE. The results demonstrated that the two substitution lines are 1D/1R, and the addition line is also 1R addition. Rye chromosomes that are distinct to the red-colored wheat chromosomes appear yellow-green at mitotic metaphase after FISH.

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