Genetic transformation of watercress with a gene encoding for betaine-aldehyde dehydrogenase (BADH)
文献类型： 外文期刊
作者： Li, YX 1 ; Chang, FQ 2 ; Du, LQ 2 ; Guo, BH 2 ; Li, HJ 2 ; Zhang, JS 2 ; Chen, SY 2 ; Zhu, ZQ 2 ;
作者机构： 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China; Beijing Normal Univ, Life Sci Coll, Beijing 100875, Peoples R China; Hebei Acad Agr & Forestry Sci, Inst Food & Oil Crops, Shijiazhuang 050031, Peoples R China; Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China
关键词： betaine-aldehyde dehydrogenase gene;transgenic plant;salt-tolerance;watercress
期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）
ISSN： 0577-7496
年卷期： 2000 年 42 卷 5 期
页码：
收录情况： SCI
摘要： Betaine-aldehyde dehydrogenase (BADH) gene was transferred into watercress ( Nasturtium officinale R. Br.) by Agrobacterium-mediated transformation. PCR and Southern blotting analysis showed that the BADH gene was integrated into genome of 46 watercress plants. Among the 6 transgenic plants being examined, 5 showed higher BADH activity than the control. Northern blotting analysis of the 5 plants showed that 4 with higher BADH activity had BADH transcript expression. Relative electronic conductivity demonstrated that less membrane damage of transgenic plants. Some transgenic plants grew normally on the medium with 0.5% NaCl while the control plants had very poor loots and slow grew in the same medium. The growth of some transgenic plants were superior to control obviously on the medium with 0.8% NaCl, although they grew slower than on the medium with 0.5% NaCl. And the leaves of control turned yellow after about 20 days to 1 month of cultured and eventually died.
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