文献类型: 外文期刊
作者: Wu, YJ 1 ; Zhao, YH 2 ; Engelmann, F 2 ; Zhou, MD 2 ; Zhang, DM 2 ; Chen, SY 2 ;
作者机构: 1.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli Town 066600, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli Town 066600, Peoples R China; IPGRI, I-00057 Rome, Italy; CAAS, IPGRI, E Asia Off, Beijing 100081, Peoples R China
关键词: Malus domestica;dormant buds;shoot tips;cryopreservation
期刊名称:CRYOLETTERS ( 影响因子:1.066; 五年影响因子:1.0 )
ISSN: 0143-2044
年卷期: 2001 年 22 卷 6 期
页码:
收录情况: SCI
摘要: Shoot tips excised from apple (Malus domestica Borkh. cv. Golden Delicious) dormant buds immediately before freezing were successfully cryopreserved using three different methods (vitrification, encapsulation-dehydration and two-step freezing). Two-step freezing produced the highest in vitro growth recovery (83%) of cryopreserved shoot tips, followed by vitrification (60%) and encapsulation-dehydration (33%). By contrast, when dormant buds were cryopreserved using the two-step freezing technique, shoot tips excised from buds after rewarming and cultured in vitro displayed only 16% recovery.
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