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Cryopreservation of apple dormant buds and shoot tips

文献类型: 外文期刊

作者: Wu, YJ 1 ; Zhao, YH 2 ; Engelmann, F 2 ; Zhou, MD 2 ; Zhang, DM 2 ; Chen, SY 2 ;

作者机构: 1.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli Town 066600, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli Town 066600, Peoples R China; IPGRI, I-00057 Rome, Italy; CAAS, IPGRI, E Asia Off, Beijing 100081, Peoples R China

关键词: Malus domestica;dormant buds;shoot tips;cryopreservation

期刊名称:CRYOLETTERS ( 影响因子:1.066; 五年影响因子:1.0 )

ISSN: 0143-2044

年卷期: 2001 年 22 卷 6 期

页码:

收录情况: SCI

摘要: Shoot tips excised from apple (Malus domestica Borkh. cv. Golden Delicious) dormant buds immediately before freezing were successfully cryopreserved using three different methods (vitrification, encapsulation-dehydration and two-step freezing). Two-step freezing produced the highest in vitro growth recovery (83%) of cryopreserved shoot tips, followed by vitrification (60%) and encapsulation-dehydration (33%). By contrast, when dormant buds were cryopreserved using the two-step freezing technique, shoot tips excised from buds after rewarming and cultured in vitro displayed only 16% recovery.

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[1]Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants. Wu, YJ,Engelmann, F,Zhao, YH,Zhou, MD,Chen, SY. 1999

[2]Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 1999

[3]Cryopreservation of apple in vitro shoot tips by the droplet freezing method. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Chen, SY. 1999

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