文献类型: 外文期刊
作者: Wang, ZN 1 ; Zhang, JS 2 ; Guo, BH 2 ; He, SJ 2 ; Tian, AG 2 ; Chen, SY 2 ;
作者机构: 1.Chinese Acad Sci, Inst Genet, Plant Biotechnol Lab, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Genet, Plant Biotechnol Lab, Beijing 100101, Peoples R China; Hebei Acad Agr & Forestry Sci, Inst Food & Oil Crops, Plant Breeding Lab, Shijiazhuang 050031, Peoples R China
关键词: wheat;Na+/H+ antiport gene;salt-inducible
期刊名称:ACTA BOTANICA SINICA ( 影响因子:0.599; )
ISSN: 0577-7496
年卷期: 2002 年 44 卷 10 期
页码:
收录情况: SCI
摘要: The Na+/H+ antiport genes named TaNHX1 and TaNHX2 were cloned by screening a salt-stressed wheat cDNA library using rice Na+/H+ antiport cDNA fragment as the probe. Sequencing analysis showed that TaNHX1 was 2 029 by in length and contained a complete ORF of 1 638 by. The TaNHX1 encodes a polypeptide of 546 amino acids with a transmembrane domain DIFFIYLLPPI. TaNHX2 was 1 693 by in length consisting of a partial ORF followed by a 3'-UTR of 808 bp. The amino acid sequence of these two genes were about 70% identical to the known NHX genes from rice, Arabidopsis and Atriplex. A RT-PCR assay showed that the level of TaNHX1 transcripts was increased and. reached a steady higher level in the seedlings after 3 h treatment with 400 mmol/L NaCl.
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