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Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants

文献类型： 外文期刊

作者： Wu, YJ 1 ; Engelmann, F 2 ; Zhao, YH 2 ; Zhou, MD 2 ; Chen, SY 2 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Hebei, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Hebei, Peoples R China; IPGRI, I-00145 Rome, Italy; CAAS, IPGRI, E Asia Off, Beijing 100081, Peoples R China

关键词： apple (Malus spp.);cryopreservation;shoot tips;two-step freezing;vitrification;encapsulation-dehydration;cold-hardening;shoot tip water content

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 1999 年 20 卷 2 期

页码：

收录情况： SCI

摘要： In this gaper, the efficiency of three techniques (two-step freezing, vitrification, encapsulation-dehydration) for freezing irt vitro shoot tips was compared and the effect of conditioning of mother-plants was investigated. Cold-hardening mother plants for 3 weeks at 5 degrees C improved regrowth rate and pattern of shoot tips whatever the cryopreservation technique employed. Increasing the time during which mother-plants were maintained on standard medium without subculture before sampling of apices decreased the water content of shoot tips from about 85-88% to 63-66% (fresh weight basis) and increased regrowth rate and pattern whatever the cryopreservation technique employed. The best results (up to 86% regrowth after cryopreservation without any callusing) were obtained with the encapsulation-dehydration technique. Using apices sampled on mother-plants which had not been subcultured for 26 weeks allowed to reduce the duration of the sucrose pregrowth treatment of encapsulated apices, thus simplifying the protocol. This simplified encapsulation-dehydration protocol was successfully applied to II out of the 12 cultivars tested.

  • 相关文献

[1]Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 1999

[2]Cryopreservation of kiwi shoot tips. Wu, YJ,Zhao, YH,Engelmann, R,Zhou, MD. 2001

[3]Cryopreservation of axillary buds of grape (Vitis vinifera) in vitro plantlets. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD. 2001

[4]Cryopreservation of apple in vitro shoot tips by the droplet freezing method. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Chen, SY. 1999

[5]Cryopreservation of apple dormant buds and shoot tips. Wu, YJ,Zhao, YH,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 2001

[6]Cryopreservation of mango (Mangifera indica L.) embryogenic cultures. Wu, YJ,Huang, XL,Xiao, JN,Li, XJ,Zhou, MD,Engelmann, F. 2003

[7]Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD. Zhai, ZY,Wu, YJ,Engelmann, F,Chen, RZ,Zhao, YH. 2003

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