河北省农林科学院机构知识库

Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD

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文献类型：外文期刊

作者： Zhai, ZY ¹ ; Wu, YJ ² ; Engelmann, F ² ; Chen, RZ ² ; Zhao, YH ² ;

作者机构： 1.Zhongshan Univ, Coll Life Sci, Guangzhou 510275, Peoples R China

2.Zhongshan Univ, Coll Life Sci, Guangzhou 510275, Peoples R China; Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli Town 066600, Peoples R China

关键词： grape;citrus;shoot-tip;cryopreservation;genetic stability;RAPD

期刊名称：CRYOLETTERS （影响因子：1.066；五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2003 年 24 卷 5 期

页码：

收录情况： SCI

摘要： In vitro cultured shoot-tips of four grape (Vitis vinifera) cultivars and one kiwi (Actinidia deliciosa) cultivar were cryopreserved using the encapsulation-dehydration method. Genomic DNA of plantlets regenerated directly from cryopreserved shoot-tips was extracted and analyzed using the RAPD (Random Amplified Polymorphic DNA) technique. The RAPD profiles obtained were highly reproducible and no differences were found between the DNA patterns obtained with plantlets regenerated from control and cryopreserved plantlets. The RAPD technique therefore appears to be a fast, simple and efficient method for evaluating genetic stability of cryopreserved material, which can be used rapidly after the completion of a freezing experiment and will efficiently complement other genetic stability evaluation methods.

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作者其他论文 更多>>

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Cryopreservation of kiwi shoot tips

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Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants

Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration

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