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Cryopreservation of apple in vitro shoot tips by the droplet freezing method

文献类型: 外文期刊

作者: Zhao, YH 1 ; Wu, YJ 2 ; Engelmann, F 2 ; Zhou, MD 2 ; Chen, SY 2 ;

作者机构: 1.IPGRI, I-00145 Rome, Italy

2.IPGRI, I-00145 Rome, Italy; CAAS, IPGRI, E Asia Off, Beijing 100081, Peoples R China; Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Hebei, Peoples R China

关键词: apple;shoot tips;cryopreservation;droplet freezing method

期刊名称:CRYO-LETTERS ( 影响因子:1.066; 五年影响因子:1.0 )

ISSN: 0143-2044

年卷期: 1999 年 20 卷 2 期

页码:

收录情况: SCI

摘要: Shoot tips sampled on in vitro plantlets of 5 apple cultivars were successfully cryopreserved using the droplet freezing method. After 3 weeks of cold-hardening at 5 degrees C, the shoot tips were precultured in liquid medium with 1.0% DMSO for 24 h at 5 degrees C, treated for 75 min. at room temperature with 0.3 M sucrose and 15% DMSO, placed in 5 mu l droplets of cryoprotectant medium containing 0.3 M sucrose and 15% DMSO on aluminium foil and cooled to -40 degrees C at 0.2 degrees C/min before immersion in liquid nitrogen. Recovery rates of cryopreserved shoot tips ranged between 70 and 92%, depending on the cultivar. Regrowth of frozen shoot tips was direct, without callus formation.

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