文献类型： 外文期刊
作者： Wu, YJ 1 ; Huang, XL 2 ; Xiao, JN 2 ; Li, XJ 2 ; Zhou, MD 2 ; Engelmann, F 2 ;
作者机构： 1.Zhongshan Univ, Minist Educ, Key Lab Gene Engn, Guangzhou 510275, Peoples R China
2.Zhongshan Univ, Minist Educ, Key Lab Gene Engn, Guangzhou 510275, Peoples R China; Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli Town 066600, Hebei, Peoples R China; CAAS, IPGRI, Subreg Off E Asia, Beijing 100081, Peoples R China; IPGRI, I-00057 Rome, Italy
关键词： Mangifera indica;embryogenic masses;vitrification;encapsulation/dehydration;pregrowth/dehydration
期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）
ISSN： 0143-2044
年卷期： 2003 年 24 卷 5 期
页码：
收录情况： SCI
摘要： Three techniques were compared for cryopreserving embryogenic masses (EMs) sampled from mango (Mangifera indica L.) cv. Zihua embryogenic cultures: (i) encapsulation/dehydration; (ii) pregrowth/dehydration; and (iii) vitrification. In all experiments, EMs were sampled from embryogenic cultures during their exponential growth phase and pretreated for 24 h on solid medium containing 0.5 M sucrose before freezing. No recovery was achieved after cryopreservation using the encapsulation/dehydration technique, whatever the moisture content (fresh weight basis) of EMs, which ranged from 78.3 % without dehydration to 40.8 % after 6 h dehydration. With the pregrowth/dehydration technique, limited recovery (8.3 %) was achieved after desiccation of EMs for I h, to 58.5 % MC. Using the vitrification technique, recovery ranged from 94.3 % after treatment of EMs with the PVS3 vitrification solution for 20 min (EM moisture content of 34.7 %) to 10.9 % after a 120 min treatment with the vitrification solution (EM moisture content of 26.0 %).
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