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Genome-wide identification of Hami melon miRNAs with putative roles during fruit development

文献类型： 外文期刊

作者： Zhang, Hong 1 ; Yin, Lan 2 ; Wang, Huaisong 3 ; Wang, Guangzhi 1 ; Ma, Xinli 1 ; Li, Meihua 1 ; Wu, Haibo 1 ; Fu, Qiushi; 1 ;

作者机构： 1.Xinjiang Acad Agr Sci, Hami Melon Res Ctr, Urumqi, Xinjiang, Peoples R China

2.ABLife Inc, Wuhan, Hubei, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 7 期

页码：

收录情况： SCI

摘要： MicroRNAs represent a family of small endogenous, non-coding RNAs that play critical regulatory roles in plant growth, development, and environmental stress responses. Hami melon is famous for its attractive flavor and excellent nutritional value, however, the mechanisms underlying the fruit development and ripening remains largely unknown. Here, we performed small RNA sequencing to investigate the roles of miRNAs during Hami melon fruit development. Two batches of flesh samples were collected at four fruit development stages. Small RNA sequencing yielded a total of 54,553,424 raw reads from eight libraries. 113 conserved miRNAs belonging to 30 miRNA families and nine novel miRNAs comprising nine miRNA families were identified. The expression of 42 conserved miRNAs and three Hami melon-specific miRNAs significantly changed during fruit development. Furthermore, 484 and 124 melon genes were predicted as putative targets of 29 conserved and nine Hami melon-specific miRNA families, respectively. GO enrichment analysis were performed on target genes, "transcription, DNA-dependent", "rRNA processing", "oxidation reduction", "signal transduction", "regulation of transcription, DNA-dependent", and "metabolic process" were the over-represented biological process terms. Cleavage sites of six target genes were validated using 5' RACE. Our results present a comprehensive set of identification and characterization of Hami melon fruit miRNAs and their potential targets, which provide valuable basis towards understanding the regulatory mechanisms in programmed process of normal Hami fruit development and ripening. Specific miRNAs could be selected for further research and applications in breeding practices.

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