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Isolation and characterization of a G9P[23] porcine rotavirus strain AHFY2022 in China

文献类型： 外文期刊

作者： Wang, Jianxin 1 ; Zhou, Jinzhu 1 ; Zhu, Xuejiao 1 ; Bian, Xianyu 1 ; Han, Nan 1 ; Fan, Baochao 1 ; Gu, Laqiang 1 ; Cheng, Xi 1 ; Li, Sufen 1 ; Tao, Ran 1 ; Li, Jizong 1 ; Zhang, Xuehan 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Peoples R China

3.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China

4.Hebei Agr Univ, Coll Vet Med, Baoding 071000, Peoples R China

5.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Anim Infect Dis & Zoonoses, Yangzhou 225009, Peoples R China

6.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Peoples R China

关键词： Porcine rotavirus; Isolation; Whole-genome sequencing; Genome analysis; Pathogenicity

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.3； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2024 年 190 卷

页码：

收录情况： SCI

摘要： Rotavirus group A (RVA) is a main pathogen causing diarrheal diseases in humans and animals. Various genotypes are prevalent in the Chinese pig herd. The genetic diversity of RVA lead to distinctly characteristics. In the present study, a porcine RVA strain, named AHFY2022, was successfully isolated from the small intestine tissue of piglets with severe diarrhea. The AHFY2022 strain was identified by cytopathic effects (CPE) observation, indirect immunofluorescence assay (IFA), electron microscopy (EM), high-throughput sequencing, and pathogenesis to piglets. The genomic investigation using NGS data revealed that AHFY2022 exhibited the genotypes G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1, using the online platform the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) (https://www.bv-brc.org/). Moreover, experimental inoculation in 5-day-old and 27- day-old piglets demonstrated that AHFY2022 caused severe diarrhea, fecal shedding, small intestinal villi damage, and colonization in all challenged piglets. Taken together, our results detailed the virological features of the porcine rotavirus G9P[23] from China, including the whole-genome sequences, genotypes, growth kinetics in MA104 cells and the pathogenicity in suckling piglets.

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