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Chromosome-level genome assembly of Qihe gibel carp

文献类型: 外文期刊

作者: Lian, Kaiqi 1 ; Shan, Jinyan 1 ; Ma, Jun 1 ; Liu, Yuling 1 ; Li, Xiaolong 1 ; Lu, Meng 2 ; Ding, Miao 2 ; Wei, Jie 4 ; Zhou, Li 2 ; Kuang, Youyi 5 ; Gui, Jianfang 2 ; Peng, Renhai 1 ;

作者机构: 1.Anyang Inst Technol, Sch Biotechnol & Food Sci, Anyang 455000, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

3.Haihe River Basin Aquat Biodivers Observat & Res S, , Linzhou, Linzhou 456576, Peoples R China

4.Tarim Univ, Coll Life Sci & Technol, Alar 843300, Xinjiang, Peoples R China

5.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

期刊名称:SCIENTIFIC DATA ( 影响因子:6.9; 五年影响因子:8.7 )

ISSN:

年卷期: 2025 年 12 卷 1 期

页码:

收录情况: SCI

摘要: Qihe gibel carp (Carassius gibelio var. Qihe) is a local population of natural gynogenetic amphitriploid (AAABBB) Carassius gibelio, and has high nutritional and economic value. In this study, we assemble a high-quality chromosome-level genome of Qihe gibel carp through DNBSEQ, PacBio HiFi, and Hi-C sequencing data. The resulting assembly consisted of 350 contigs with the full length of 1.607 Gb and 96.21% (1.515 Gb) of the assembled genome was successfully anchored to 50 chromosomes, with a contig N50 of 28.97 Mb and a scaffold N50 of 29.84 Mb. Repeated sequences accounting for 43.72% (732.494 Mb) of the total were also identified, and gene prediction revealed 46,131 protein-coding genes with an annotation ratio of 96.48%. Furthermore, Benchmarking Universal Single-Copy Orthologue (BUSCO) analysis demonstrated that the genome assembly achieved high completeness, with a score of 97.66%. This high-quality chromosome-level genome lays the foundation for molecular biology research as well as molecular breeding and evolutionary studies of Qihe gibel carp in the future.

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