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Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet

文献类型: 外文期刊

作者: Zhou, Chuanpeng 1 ; Lin, Heizhao 2 ; Huang, Zhong 2 ; Wang, Jun 1 ; Wang, Yun 1 ; Yu, Wei 2 ;

作者机构: 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab Aquat Prod Proc, Guangzhou 510300, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen 518121, Peoples R China

关键词: Trachinotus ovatus;Carbohydate;Glucokinase;Gene cloning;Enzyme activity;Gene expression

期刊名称:AQUACULTURE ( 影响因子:4.242; 五年影响因子:4.723 )

ISSN: 0044-8486

年卷期: 2018 年 485 卷

页码:

收录情况: SCI

摘要: It is well known that the enzyme glucokinase (GK) plays an important role in the control of glucose homeostasis. In the present study, a glucokinase (To-GK) was cloned from golden pompano, Trachinotus ovatus. The To-GK cDNA is composed of 2151 bp with a 33 bp of 5'-UTR, 1437 bp open reading frame (ORF) and 681 bp 3'-UTR, encoding a polypeptide of 478 amino acids (GenBank accession no: ALX36181.1). Phylogenetic analysis revealed that To-GK showed highest similarity to Cynoglossus semilaevis GK. Tissue distribution analysis showed that the highest expression of the To-GK was detected in liver. Then, 8-week feeding trial was conducted to investigate the effects of dietary carbohydrate levels (57.5, 120.4, and 277.7 g kg(-1)) on the activity and mRNA expression of To-GK in T. ovatus. Higher GK activity was found in fish fed 277.7 g kg-1 carbohydrate diet. To-GK expression was higher in fish fed the 277.7 g kg(-1) carbohydrate diet. Overall, our data suggest that the hepatic GK activity and mRNA expression of T. ovatus were up-regulated significantly as dietary carbohydrate levels increased. These results indicated that To-GK plays a crucial role in the adaptation of hepatic glycolysis to dietary high carbohydrates in T. ovatus.

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