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Generation of all-male-like sterile zebrafish by eliminating primordial germ cells at early development

文献类型： 外文期刊

作者： Zhou, Li 1 ; Fang, Yongyong 1 ; Wang, Fang 1 ; Dong, Xiaohua 2 ; Jiang, Lan 1 ; Liu, Chun 1 ; Zhao, Qinshun 2 ; Li, Kaibi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, 1 Xingyu Rd, Guangzhou 510380, Guangdong, Peoples R China

2.Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Pukou Hightech Dev Zone, 12 Xuefu Rd, Nanjing 210061, Jiangsu, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： Production of all-male and sterile fish may not only substantially improve yield but also be crucial for the application of genome modified species in aquaculture. Previously, it was reported that the fish lacking primordial germ cells (PGCs) becomes infertile, and nitroreductase, an enzyme converting non-toxic metronidazole (MTZ) into toxic metabolites, induces targeted toxicity to kill the cells expressing it. In this study, we generated a transgenic zebrafish line of Tg(nanos3:nfsB-mCherry-nanos3 3' UTR) in which the NfsB nitroreductase is solely expressed in PGCs. Treating the embryos derived from the female transgenic zebrafish with MTZ from 0 through 2 dpf (days post fertilization), we found that the germ cells were completely eliminated in the ones older than 2.5 dpf. At 20 dpf, the MTZ-treated juvenile had no germ cells in their gonads. At 100 dpf, the MTZ-treated adult exhibited male-like morphology and showed normal mating behaviors although they had no germ cells but only supporting cells in their gonads. Taken together, our results demonstrated that conditional elimination of PGCs during early development make the zebrafish male-like and infertile. It may provide an alternative strategy to make sterile and all-male farmed fish that is good for increasing aquaculture yield and preventing the genome modified species from potential ecological risks.

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