您好,欢迎访问中国水产科学研究院 机构知识库!

The protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish (Takifugu obscurus) under low temperature stress

文献类型: 外文期刊

作者: Cheng, Chang-Hong 1 ; Liang, Hai-Yan 2 ; Luo, Sheng-Wei 2 ; Wang, An-Li 2 ; Ye, Chao-Xia 2 ;

作者机构: 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr,Key Lab Aquat Prod Proc, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China

2.South China Normal Univ, Sch Life Sci, Guangdong Higher Educ Inst,Key Lab Ecol & Environ, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Guangzhou 510631, Guangdong, Peoples R China

关键词: Takifugu obscurus;Vitamin C;Comparative proteomics;Cold stress

期刊名称:JOURNAL OF THERMAL BIOLOGY ( 影响因子:2.902; 五年影响因子:3.102 )

ISSN: 0306-4565

年卷期: 2018 年 71 卷

页码:

收录情况: SCI

摘要: The aim of this study was to investigate the protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish under low temperature stress. Six diets were formulated to contain 2.60, 48.90, 95.50, 189.83, 382.40, 779.53 mg/kg vitamin C. After 8-week feeding trial, fish were exposed to low temperature challenge. The results showed that pufferfish receiving vitamin C diet exhibited a significant decrease in ROS production (48.9-189.83 mg/kg vitamin C diet groups), cytoplasmic free-Ca2+ concentration (48.9-779.53 mg/kg vitamin C diet groups), apoptotic cell ratio (95.5-779.53 mg/kg vitamin C diet groups) and DNA damage (189.83-779.53 mg/kg vitamin C diet groups) under low temperature stress in comparison with those of control. We also investigated the alteration in protein expression under low temperature stress by a comparative proteomic analysis. The results demonstrated that 24 protein spots showed significantly differential expression in the cold-stress-treated group compared with those of the control group, and 5 protein spots were successfully identified. Furthermore, comparative proteomic analysis revealed that vitamin C could increase expressed proteins related to energy metabolism, immune-responses and cytoskeleton. These findings would be helpful to understand the protective effects of vitamin C against cold stress.

  • 相关文献

[1]Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress. Cheng, Chang-Hong,Guo, Zhi-Xun,Ye, Chao-Xia,Wang, An-Li,Guo, Zhi-Xun.

[2]Effects of high temperature on biochemical parameters, oxidative stress, DNA damage and apoptosis of pufferfish (Takifugu obscurus). Cheng, Chang-Hong,Guo, Zhi-Xun,Luo, Sheng-Wei,Wang, An-Li,Guo, Zhi-Xun. 2018

[3]Molecular and immune response characterizations of a novel Bax inhibitor-1 gene in pufferfish, Takifugu obscurus. Cheng, Chang-Hong,Guo, Zhi-Xun,Luo, Sheng-Wei,Wang, An-Li,Guo, Zhi-Xun.

[4]Effect of dietary astaxanthin on the growth performance, non-specific immunity, and antioxidant capacity of pufferfish (Takifugu obscurus) under high temperature stress. Cheng, Chang-Hong,Guo, Zhi-Xun,Cheng, Chang-Hong,Guo, Zhi-Xun,Ye, Chao-Xia,Wang, An-Li,Ye, Chao-Xia,Wang, An-Li. 2018

[5]Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of obscure puffer (Takifugu obscurus) and cross-species amplification. Ma, Hongyu,Chen, Songlin,Liao, Xiaolin,Xu, Tianjun,Ma, Hongyu,Ge, Jiachun.

[6]Transcriptome analysis reveals the potential mechanism of the albino skin development in pufferfish Takifugu obscurus. Jin, Wu,Wen, Haibo,Gu, Ruobo,Jin, Wu,Du, Xingwei,Zheng, Jinliang.

[7]Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces). Li, Ping,Hulak, Martin,Rodina, Marek,Li, Zhi-Hua,Linhart, Otomar,Li, Ping,Li, Zhi-Hua,Sulc, Miroslav.

[8]Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress. Ming, Jianhua,Xie, Jun,Xu, Pao,Ge, Xianping,Ming, Jianhua,Ye, Jinyun,Ming, Jianhua,Liu, Wenbin. 2012

[9]Effects of Dietary Vitamin C on the Physiological Responses and Disease Resistance to Ph Stress and Aeromonas Hydrophila Infection of Megalobrama Amblycephala. Liu, Bo,Wan, Jinjuan,Ge, Xianping,Xie, Jun,Liu, Bo,Ge, Xianping,Xie, Jun,Zhou, Qunlan,Miao, Linghong,Ren, Mingchun,Pan, Liangkun. 2016

[10]Vitamin C requirement of adult genetically improved farmed tilapia, Oreochromis niloticus. Wu, Fan,Huang, Feng,Wen, Hua,Jiang, Ming,Liu, Wei,Tian, Juan,Yang, Chang Geng.

[11]Dietary vitamin C requirement of genetically improved farmed Tilapia, Oreochromis Niloticus. Huang, Feng,Jiang, Ming,Wen, Hua,Wu, Fan,Liu, Wei,Tian, Juan,Shao, Hui,Jiang, Ming,Tian, Juan.

[12]iTRAQ-based quantitative proteomics reveals the biochemical mechanism of cold stress adaption of razor clam during controlled freezing-point storage. Wang, Chong,Chu, Jianjun,Fu, Linglin,Wang, Yanbo,Zhao, Feng,Zhou, Deqing. 2018

[13]Isolation and Characterization of a Stress-Dependent Plastidial Delta(12) Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ-7. Lu, Yandu,Qin, Song,Lu, Yandu,Li, Fuchao,Liu, Shaofang,Lu, Yandu,Liu, Shaofang,Chi, Xiaoyuan,Yang, Qingli,Li, Zhaoxin,Gan, Qinhua. 2010

[14]Changes in the fatty acid composition and regulation of antioxidant enzymes and physiology of juvenile genetically improved farmed tilapia Oreochromis niloticus (L.), subjected to short-term low temperature stress. He, J.,Xu, P.,Yang, R. Q.,He, J.,Qiang, J.,Yang, H.,Xu, P.,Zhu, Z. X..

[15]Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7. Lu, Yandu,Qin, Song,Lu, Yandu,Liu, Shaofang,Lu, Yandu,Liu, Shaofang,Chi, Xiaoyuan,Yang, Qingli,Li, Zhaoxin,Gan, Qinhua.

作者其他论文 更多>>