The protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish (Takifugu obscurus) under low temperature stress
文献类型: 外文期刊
作者: Cheng, Chang-Hong 1 ; Liang, Hai-Yan 2 ; Luo, Sheng-Wei 2 ; Wang, An-Li 2 ; Ye, Chao-Xia 2 ;
作者机构: 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr,Key Lab Aquat Prod Proc, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China
2.South China Normal Univ, Sch Life Sci, Guangdong Higher Educ Inst,Key Lab Ecol & Environ, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Guangzhou 510631, Guangdong, Peoples R China
关键词: Takifugu obscurus;Vitamin C;Comparative proteomics;Cold stress
期刊名称:JOURNAL OF THERMAL BIOLOGY ( 影响因子:2.902; 五年影响因子:3.102 )
ISSN: 0306-4565
年卷期: 2018 年 71 卷
页码:
收录情况: SCI
摘要: The aim of this study was to investigate the protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish under low temperature stress. Six diets were formulated to contain 2.60, 48.90, 95.50, 189.83, 382.40, 779.53 mg/kg vitamin C. After 8-week feeding trial, fish were exposed to low temperature challenge. The results showed that pufferfish receiving vitamin C diet exhibited a significant decrease in ROS production (48.9-189.83 mg/kg vitamin C diet groups), cytoplasmic free-Ca2+ concentration (48.9-779.53 mg/kg vitamin C diet groups), apoptotic cell ratio (95.5-779.53 mg/kg vitamin C diet groups) and DNA damage (189.83-779.53 mg/kg vitamin C diet groups) under low temperature stress in comparison with those of control. We also investigated the alteration in protein expression under low temperature stress by a comparative proteomic analysis. The results demonstrated that 24 protein spots showed significantly differential expression in the cold-stress-treated group compared with those of the control group, and 5 protein spots were successfully identified. Furthermore, comparative proteomic analysis revealed that vitamin C could increase expressed proteins related to energy metabolism, immune-responses and cytoskeleton. These findings would be helpful to understand the protective effects of vitamin C against cold stress.
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