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Physiological responses and gene expression changes in the western mosquitofish (Gambusia affinis) exposed to progesterone at environmentally relevant concentrations

文献类型: 外文期刊

作者: Hou, Liping 1 ; Xu, Hongyan 2 ; Ying, Guangguo 3 ; Yang, Yang 1 ; Shu, Hu 1 ; Zhao, Jianliang 3 ; Cheng, Xuemei 1 ;

作者机构: 1.Guangzhou Univ, Sch Life Sci, Guangzhou 510655, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

3.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China

4.South China Normal Univ, Environm Res Inst, MOE Key Lab Environm Theoret Chem, Guangzhou 510006, Guangdong, Peoples R China

关键词: Progesterone;Fish;Masculinization;Endocrine disruption;Histopathology;Gene expression

期刊名称:AQUATIC TOXICOLOGY ( 影响因子:4.964; 五年影响因子:5.071 )

ISSN: 0166-445X

年卷期: 2017 年 192 卷

页码:

收录情况: SCI

摘要: Progesterone (P4) is a natural and synthetic steroid, widely distributed in the aquatic environments. It can lead to adverse effects on the endocrine system in aquatic organisms. This study investigated the toxicological effects of exposure to environmentally relevant concentrations (4, 44, and 410 ng/L) of progesterone for 42 d on adult female mosquitofish, Gambusia affinis. We performed morphological and histological analyses on gonads, anal fins, liver, and gills after the exposure of mosquito fish to P4. The expression levels of genes (vtg, er, and ar isoforms) related to fish reproduction and detoxification (cyp1a) in the liver were quantified by quantitative real-time polymerase chain reaction. The results showed that the progesterone exposure induced slight masculinization in female mosquitofish, influenced the oocyte maturation as revealed by histology of the ovaries, and caused severe damages to the liver and gills of adult female mosquitofish. It also suppressed the mRNAs expression of vtg, er, cyp1a, and significantly enhanced the expression of ar mRNA in the liver. This study reveals the molecular and physiological effects of progesterone at environmentally relevant concentrations, which might further be translated to alterations in the reproduction of mosquitofish.

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