文献类型： 外文期刊

作者： Peng, S. M. ¹ ; Zhang, C. J. ¹ ; Gao, Q. X. ¹ ; Shi, Z. H. ¹ ; Chen, C. ² ; Wang, J. G. ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

3.Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China

期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）

ISSN： 0175-8659

年卷期： 2017 年 33 卷 4 期

页码：

收录情况： SCI

摘要： Nine test diets were formulated at three low levels of crude protein (P; 35%, 40% or 45%) and three high levels of crude lipid (L; 9%, 12% or 15%) to investigate whether Epinephelus moara differs in the nutrient requirements from any of the other closely-related grouper species, and if there are any indications that a further decrease in protein with increasing dietary lipid would induce a higher protein-sparing effect. Fish (20.34 +/- 0.82g/ind) were distributed among 27 experimental cages (100cm diameter, 80cm depth), with 20 fish per cage and three replicates for each test diet. The experiment was conducted over an 8-week period at a temperature of 23.9-25.7 degrees C. Weight gain and specific growth rate (SGR) tended to increase either with an increase in dietary protein level (at the same lipid level) or with the increase in dietary lipid level (at the same protein level, except at the 45% protein level). The highest weight gain and SGR occurred in fish fed the diet P45/L12. The serum cholesterol, high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) contents in fish were significantly increased as the dietary lipid increased. The increase of dietary protein significantly decreased the activities of serum and hepatic alanine aminotransferase (ALT) as well as aspartate aminotransferase (AST). Hepatic fatty acid synthase (FAS) activity significantly increased with the decrease of dietary protein and lipid levels (p<.05). However, lipoprotein lipase (LPL) activity showed an inverse trend compared to FAS. Hepatic glutamate dehydrogenase (GDH) activity significantly increased with the increase in the dietary protein level. In conclusion, the results reveal that growth performance and metabolic responses of E.moara are dependent on dietary protein and lipid levels, and that protein-sparing action can be induced by increasing the dietary lipid. To support the feeding and growth of juvenile E.moara under the present conditions, 45% protein and 12% lipid are adequate.