文献类型： 外文期刊
作者： Tian, Y. S. 1 ; Chen, S. L. 1 ; Ji, X. S. 2 ; Zhai, J. M. 3 ; Sun, L. J. 3 ; Chen, C. 1 ; Su, P. Z. 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China
2.Shandong Agr Univ, Tai An 271018, Shandong, Peoples R China
3.Shandong Mingbo Fisheries Co Ltd, Laizhou 266031, Peoples R China
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
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收录情况： SCI
摘要： Spotted halibut (Verasper variegatus) is now regarded as being endangered in China because of its continuous decline in the last three decades. Preservation and protection of its genetic resources were demanded in order to protect this rare fish species. In this project, cryopreservation technique for sperm of spotted halibut was developed. The effects of various extenders and cryoprotectants on motility of frozen-thawed sperm were examined. The motility of frozen-thawed sperm in TS-2 was higher than that in ASW and MPRS (p <0.05) and was not significantly different from that of fresh sperm (p >0.05). While the motility of frozen-thawed sperm cryopreserved with 13.3% EG (ethylene glycol), 13.3% glycerol, 13.3% MeOH (methanol) and 13.3% DMF (dimethylformamide) was less than 5%, no significant differences were observed in the motility between fresh sperm and frozen-thawed sperm cryopreserved with 13.3% DMSO (dimethyl sulfoxide), 13.3% PG (propylene glycol) (p >0.05). Using the above method, we cryopreserved spotted halibut semen with extender TS-2 and 13.3% DMSO or 13.3% PG. As a result, the fertilization rate (34.52pl10.92%) and hatching rate (23.53pl11.80%) of frozen-thawed sperm were not significantly different from that of fresh sperm (p >0.05). Motility and time delay in the activation of frozen-thawed sperm activated by artificial seawater at different salinity were different. Low salinity (low osmolality) could delay the activation of frozen-thawed sperm. The highest motility was observed with artificial seawater of 30[per thousand] (p <0.05). The most suitable temperature of seawater to activate spotted halibut frozen sperm was determined to be 18 pC (p <0.05). However, temperature of seawater has no significant effect on time delay in the activation of frozen-thawed sperm (p >0.05).
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