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Molecular identification and functional analysis of Ctrp9 in Epinephelus coioides

文献类型： 外文期刊

作者： Yang, Guokun 1 ; Qin, Chaobin 2 ; Wang, Bin 3 ; Jia, Jirong 1 ; Yuan, Xi 1 ; Li, Wensheng 1 ;

作者机构： 1.Sun Yat Sen Univ, Sun Yat Sen Univ Shen Zhen, Inst Aquat Econ Anim, State Key Lab Biocontrol,Res Inst,Sch Life Sci, Guangzhou, Guangdong, Peoples R China; Sun Yat Sen Univ, Sun Yat Sen Univ Shen Zhen, Guangdong Prov Key Lab Aquat Econ Anim, Res Inst,Sch Life Sci,South China Sea Bioresource, Guangzhou, Guangdong, Peoples R China

2.Henan Normal Univ, Coll Fisheries, Engn Technol Res Ctr Henan Prov Aquat Anim Cultiv, Xinxiang, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao, Peoples R China

关键词： Ctrp9;food intake;metabolism;grouper

期刊名称：JOURNAL OF MOLECULAR ENDOCRINOLOGY （ 影响因子：5.098； 五年影响因子：4.755 ）

ISSN： 0952-5041

年卷期： 2017 年 58 卷 4 期

页码：

收录情况： SCI

摘要： CTRP9 is a member of the C1q/TNF-related protein (CTRP) superfamily and has been studied in mammals, whereas the comparative studies of CTRP9 in non-mammalian species are still absent. In this study, ctrp9 was isolated and characterized from the orange-spotted grouper (Epinephelus coioides). The full-length cDNA of ctrp9 was 1378 bp in size with an ORF (open reading frame) of 1020 bp that encodes a 339 amino acid pre-pro hormone. The mRNA expression of ctrp9 showed a rather high level in the kidney and brain, but a low level in other tissues. Furthermore, the mRNA expression of ctrp9 decreased significantly in the liver after fasting for 7 days and restored to the normal levels after refeeding. In contrast, the ctrp9 mRNA level increased in the hypothalamus after fasting. The recombinant gCtrp9 (globular Ctrp9) was prepared using the Pichia pastoris expression system and was verified by Western blot as well as mass spectrometry assays. In the primary hepatocytes culture, the recombinant gCtrp9 could inhibit the glucose production after 12-h treatment. After i.p. (intraperitoneal) injection with recombinant gCtrp9, in hypothalamus, mRNA expression levels of npy and orexin (orexigenic factors) decreased, whereas the expression levels of crh and pomc (anorexigenic factors) increased. Moreover, i.p. injection with the recombinant gCtrp9 could reduce the serum concentrations of glucose, TG and low-density lipoprotein cholesterol but increase the content of high-density lipoprotein cholesterol. Our studies for the first time unveil the structure of Ctrp9 and its potential role as a regulatory factor of metabolism and food intake in teleost.

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