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Cloning, characterization, and expression of a novel member of proteasomal subunits gene in turbot, Scophthalmus maximus

文献类型： 外文期刊

作者： Zhang Bo 1 ; Wang Xianli 2 ; Song Wenping 1 ; Zheng Debin 1 ; Ma Chao 1 ; Xiao Guangxia 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Bohai Sea Fisheries Res Ctr, Tianjin Bohai Sea Fisheries Res Inst, Tianjin 300457, Peoples R China

2.Tongji Univ Sch Med, Tongji Hosp, Translat Ctr Stem Cell Res, Stem Cell Res Ctr, Shanghai 200065, Peoples R China

关键词： major histocompatibility complex (MHC);LMP7;qRT-PCR;immune response;turbot;Vibrio anguillarum

期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2015 年 34 卷 1 期

页码：

收录情况： SCI

摘要： The proteasome is a large, polymeric protease complex responsible for the degradation of intracellular proteins and generation of peptides that bind to class I major histocompatibility complex (MHC) molecules. This study identified a new member of proteasomal subunits in turbots (Scophthalmus maximus). The fulllength cDNA sequence of turbot proteasomal subunit was obtained. Sequence analysis indicated that its primary structure is highly similar to that of LMP7 from other vertebrates. The relationship between the turbot LMP7 expression and immune responses to pathogen infection was reported. Quantitative reverse transcriptase polymerase chain reaction showed that LMP7 was expressed differently in various tissues, with higher expression in the spleen, liver, muscle, and skin. The LMP7 expression was the highest at 96 h after challenge with lymphocyctis disease virus (LCDV) and at 12 h after challenge with Vibrio anguillarum in the turbot liver, kidney, and spleen. Furthermore, the LMP7 expression distinctly increased in turbot kidney cells at 24 h after challenge with V. anguillarum and at 96 h after challenge with LCDV. These results indicate that the turbot LMP7 protein participates in immune responses and may play a significant role in the immune process.

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