Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiae by pre-absorbed immunoproteomics
文献类型: 外文期刊
作者: Liu, Guangjin 1 ; Zhang, Wei 1 ; Liu, Yongjie 1 ; Yao, Huochun 1 ; Lu, Chengping 1 ; Xu, Pao 2 ;
作者机构: 1.Nanjing Agr Univ, Key Lab Anim Bacteriol, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R China
关键词: Streptococcus agalactiae;Piscine;Pre-absorbed immunoproteomic method (PAIM);Serine-rich repeat protein (Srr);Zebrafish
期刊名称:BMC VETERINARY RESEARCH ( 影响因子:2.741; 五年影响因子:2.955 )
ISSN: 1746-6148
年卷期: 2014 年 10 卷
页码:
收录情况: SCI
摘要: Background: Since 2009, large-scale Streptococcus agalactiae infections have broken out in cultured tilapia farms in China, resulting in considerable economic losses. Screening of the surface proteins is required to identify virulence factors or protective antigens involved in piscine S. agalactiae infections in tilapia. Pre-absorbed immunoproteomics method (PAIM) is a useful method previously established in our laboratory for identifying bacterial surface proteins. Results: A serine-rich repeat protein family 1 (Srr-1), designated XF, was identified by PAIM in piscine S. agalactiae isolate GD201008-001. To investigate the role of XF in the pathogenesis of piscine S. agalactiae, an isogenic xf mutant strain (Delta xf) and a complemented strain (C Delta xf) were successfully constructed. The Delta xf mutant and C Delta xf showed no significant differences in growth characteristics and adherence to HEp-2 cells compared with the wild-type strain. However the 50% lethal dose of Delta xf was increased (4-fold) compared with that of the parental strain in a zebrafish infection model. Conclusions: The findings demonstrated that XF is a virulence-related, highly immunoreactive surface protein and is involved in the pathogenicity of S. agalactiae infections in fish.
- 相关文献
作者其他论文 更多>>
-
HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide (Leuciscus waleckii) Under Extreme Alkaline Conditions
作者:Zhao, Xuefei;Zhang, Yu;Li, Shuqi;Bai, Suying;Zhang, Wei;Xu, Yanchun;Zhao, Xuefei;Bai, Suying;Zhang, Wei;Xu, Yanchun;Zhao, Xuefei;Chang, Yumei
关键词:ammonia excretion;
Leuciscus waleckii ; HIF1A; RHBG; alkali tolerant fish -
Genetic parameter estimation of tolerance traits for ammonia-nitrogen and nitrite-nitrogen in Urechis unicinctus
作者:Zhang, Wei;Wang, Kai;Ma, Yanqing;Lai, Wenyuan;Shao, Mingyu;Zhao, Yongzheng;Wang, Hongtao;Zhang, Zhifeng;Ma, Yubin;Zhang, Zhifeng;Sui, Juan;Chen, Zongtao
关键词:Ammonia-nitrogen; Nitrite-nitrogen; Genetic parameters; Selective breeding; Urechis unicinctus
-
Comparative Genomics of Bryopsis hypnoides: Structural Conservation and Gene Transfer Between Chloroplast and Mitochondrial Genomes
作者:Liu, Ziwen;Fan, Xiao;Wu, Yukun;Zhang, Wei;Zhang, Xiaowen;Xu, Dong;Wang, Yitao;Sun, Ke;Wang, Wei;Ye, Naihao;Liu, Ziwen;Fan, Xiao;Ye, Naihao
关键词:chloroplast genome; mitochondrial genome; gene transfer
-
Estimation of genetic parameters for the alkalinity tolerance trait in Urechis unicinctus
作者:Zhang, Wei;Wang, Kai;Shao, Mingyu;Zhao, Yongzheng;Wang, Hongtao;Zhang, Zhifeng;Ma, Yubin;Zhang, Zhifeng;Sui, Juan;Chen, Zongtao
关键词:Alkali tolerance; Genetic parameters; Selective breeding; Urechis unicinctus
-
Multi-omics and biochemical analyses provide insights into hepatic glucolipid metabolism in red tilapia ( Oreochromis spp.) under salinity stress
作者:Jiang, Bingjie;Tao, Yifan;Lu, Siqi;Hua, Jixiang;Li, Yan;Dong, Yalun;Xu, Pao;Qiang, Jun;Huang, Renshan;Xu, Pao;Qiang, Jun
关键词:Glucolipid metabolism; Multi-omics; Physiological response; Red tilapia; Salinity stress
-
Genome-Wide Association Study and RNA-Seq Analysis Uncover Candidate Genes Controlling Growth Traits in Red Tilapia (Oreochromis spp.) Under Hyperosmotic Stress
作者:Jiang, Bingjie;Tao, Yifan;Lu, Siqi;Xu, Pao;Qiang, Jun;Liu, Kai;Tao, Wenjing;Badran, Mohamed Fekri;Saleh, Moustafa Hassan Lotfy;Aboueleila, Rahma Halim Mahmoud;Xu, Pao;Qiang, Jun;Liu, Kai
关键词:red tilapia; growth traits; RNA-seq; GWAS; candidate gene
-
Immune response of intestinal micrornas to transport stress in hybrid yellow catfish (tachysurus fulvidraco♀ xt. vachellii♂) : response strategy and potential biomarker
作者:Zheng, Tao;Zheng, Tao;Qiang, Jun;Xu, Pao;Zheng, Tao;Tao, Yifan;Lu, Siqi;Qiang, Jun;Xu, Pao
关键词:Transport stress; Intestine; Immune regulation; Hybrid yellow catfish; RNA sequencing



