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The Transcriptomes of the Crucian Carp Complex (Carassius auratus) Provide Insights into the Distinction between Unisexual Triploids and Sexual Diploids

文献类型: 外文期刊

作者: Li, Chun-Yan 1 ; Li, Jiong-Tang 3 ; Kuang, You-Yi 1 ; Xu, Ru 3 ; Zhao, Zi-Xia 3 ; Hou, Guang-Yuan 3 ; Liang, Hong-Wei; 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150001, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China

4.Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China

5.Dalian Ocean Univ, Col

关键词: crucian carp;gibel carp;RNA-seq;genetic diversity;unisexual reproduction

期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )

ISSN: 1422-0067

年卷期: 2014 年 15 卷 6 期

页码:

收录情况: SCI

摘要: Both sexual reproduction and unisexual reproduction are adaptive strategies for species survival and evolution. Unisexual animals have originated largely by hybridization, which tends to elevate their heterozygosity. However, the extent of genetic diversity resulting from hybridization and the genomic differences that determine the type of reproduction are poorly understood. In Carassius auratus, sexual diploids and unisexual triploids coexist. These two forms are similar morphologically but differ markedly in their modes of reproduction. Investigation of their genomic differences will be useful to study genome diversity and the development of reproductive mode. We generated transcriptomes for the unisexual and sexual populations. Genes were identified using homology searches and an ab initio method. Estimation of the synonymous substitution rate in the orthologous pairs indicated that the hybridization of gibel carp occurred 2.2 million years ago. Microsatellite genotyping in each individual from the gibel carp population indicated that most gibel carp genes were not tri-allelic. Molecular function and pathway comparisons suggested few gene expansions between them, except for the progesterone-mediated oocyte maturation pathway, which is enriched in gibel carp. Differential expression analysis identified highly expressed genes in gibel carp. The transcriptomes provide information on genetic diversity and genomic differences, which should assist future studies in functional genomics.

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