A 66-bp deletion in growth hormone releasing hormone gene 5 '-flanking region with largemouth bass recessive embryonic lethal
文献类型： 外文期刊
作者： Ma, D. M. 1 ; Han, L. Q. 1 ; Bai, J. J. 1 ; Li, S. J. 1 ; Fan, J. J. 1 ; Yu, L. Y. 2 ; Quan, Y. C.;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr Peoples Republ China, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr Peoples Republ China
关键词： recessive lethal;livability;homozygous lethal;Micropterus salmoides;indel site
期刊名称：ANIMAL GENETICS （ 影响因子：3.169； 五年影响因子：3.058 ）
ISSN： 0268-9146
年卷期： 2014 年 45 卷 3 期
页码：
收录情况： SCI
摘要： Growth hormone releasing hormone (GHRH) regulates the secretion of growth hormone (GH) in the pituitary gland. A 66-bp deletion (c.-923_-858del) was detected in the 5 '-flanking sequence of the largemouth bass (Micropterus salmoides) GHRH gene. In two cultured random populations of adult individuals (A: n=170 and B: n=150), the genotype ratios of +/+:+/- were 2.5:1 and 2.8:1 respectively. Only one -/- fish was detected. A Largemouth bass family was constructed with two heterozygous individuals (+/-) as parents. The genotype ratio of +/+:+/-:-/- in the filial generation embryos was 1:1.6:0.1 at the neurula and 1:2:0 at hatched larvae stages. This indicated that the 66-bp deletion was a recessive lethal site and that homozygous individuals (-/-) died off in embryonic development. The growth traits (body weight, body length and body depth) were measured, and the GHRH mRNA expression levels in brain tissue were detected using real-time PCR. The effects of genotype (+/-) on growth traits and GHRH mRNA expression were not significant. Although the cause of death was not clear, the results hint that the 66-bp deletion site in GHRH 5 '-flanking sequence significantly affects the livability in largemouth bass embryonic development.
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