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Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery

文献类型: 外文期刊

作者: Yu, L. Y. 1 ; Bai, J. J. 1 ; Fan, J. J. 1 ; Ma, D. M. 1 ; Quan, Y. C. 1 ; Jiang, P. 1 ;

作者机构: 1.Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou, Guangdong, Peoples R China; Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China

关键词: Transcriptome analysis;Grass carp;Pyrosequencing;Molecular marker

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2015 年 14 卷 4 期

页码:

收录情况: SCI

摘要: Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after BLAST. Of these, 24,010 (64.74%) were annotated in the National Center for Biotechnology Information database, and 3715 simple sequence repeats and 2008 single nucleotide polymorphisms were identified in this EST dataset as potential molecular markers. This study provides new data for functional genomic and biological research on grass carp. The markers identified in this study will enrich the currently used molecular markers and facilitate marker-assisted selection in grass carp-breeding programs. These results also demonstrate that transcriptomic analysis based on 454 sequencing is a powerful tool for gene discovery and molecular marker development in non-model species.

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