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White spot syndrome virus VP12 interacts with adenine nucleotide translocase of Litopenaeus vannamei

文献类型: 外文期刊

作者: Ma, Fang-fang 1 ; Chou, Zhi-guang 1 ; Liu, Qing-hui 1 ; Guan, Guangkuo 1 ; Li, Chen 1 ; Huang, Jie 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Shanghai, Peoples R China

关键词: VP12;Adenine nucleotide translocase;White spot syndrome virus;Litopenaeus vannamei

期刊名称:JOURNAL OF INVERTEBRATE PATHOLOGY ( 影响因子:2.841; 五年影响因子:3.368 )

ISSN: 0022-2011

年卷期: 2014 年 118 卷

页码:

收录情况: SCI

摘要: White spot syndrome virus VP12 contains cell attachment motif RGD which is considered to be critical for host cell binding. Until now, the function of this protein remains undefined. In this study, we explored the interaction of VP12 with host cells. A new shrimp protein (adenine nucleotide translocase of Litopenaeus vannamei, LvANT) is selected by far-western overlay assay. Tissue distribution of adenine nucleotide translocase mRNA showed that it was commonly spread in all the tissues detected. Cellular localization of LvANT in shrimp hemocytes showed that it was primarily located in the cytoplasm of hemocytes and colocalized with mitochondria. ELISA and far-western blot assay confirmed that VP12 interacted with LvANT. In vivo neutralization assay showed that anti-LvANT antibody can significantly reduce the mortality of shrimp challenged by WSSV at 48 h post-treatment. Our results collectively showed that VP12 is involved in host cell binding via interaction with adenine nucleotide translocase. (C) 2014 Elsevier Inc. All rights reserved.

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