您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Establishment of a Sensitive Nitric Oxide Bioassay for Bioscreening Application

文献类型： 外文期刊

作者： Yang, Qiao 1 ; Zhang, Xiaoling 1 ; Huang, Hongliang 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab East China Sea & Ocean Fishery Resources, Sci Observing & Expt Stn Fisheries Resources & En, East China Sea Fisheries Res Inst, Wuxi, Jiangsu, Peoples R China

关键词： NO oxide;Bioassay;High-throughput;Bioscreening

期刊名称：ADVANCES IN MATERIALS AND MATERIALS PROCESSING IV, PTS 1 AND 2

ISSN： 1022-6680

年卷期： 2014 年 887-888 卷

页码：

收录情况： SCI

摘要： Nitric oxide is a free-radical diatomic molecule and an important biologic messenger molecule involved in the regulation of diverse physiological and pathophysiological mechanisms in the cardiovascular system, the central and peripheral nervous systems and the immune system. The discovery of NO with these important physiological functions has led to the development of various analytical methods for its detection and quantification. The involvement of nitric oxide in a plethora of vital biological functions makes its detection and quantification of great interest. Among various methods of measurement of the production of nitric oxide in cellular systems, those based on the use of fluorogenic probes have been gaining popularity due to their simplicity and sensitivity. BODIPY has advantages of high extinction coefficients, high fluorescence quantum efficiency and stability to light. Moreover, its emission wavelength can be adjusted by modifying chemical groups. Our lab has been exploring functionalized DABs for labeling and detection of NO several years before. This study was aimed at an evaluation of the specificity of reaction of DAMBO-P-H with NO, in particular at an assessment of its reactivity with nitric oxide and its bioscreening application to biological systems.

  • 相关文献

[1]Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-throughput Microplate Format. Zhang, Xiaoling,Yang, Qiao. 2013

[2]Development of the Reliable Fluorometric Assay for the Sensitive Determination of Nitric Oxide Metabolites in Biological Fluids. Yang, Qiao,Zhang, Xiaoling,Huang, Hongliang. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台