Development of the Reliable Fluorometric Assay for the Sensitive Determination of Nitric Oxide Metabolites in Biological Fluids
文献类型： 外文期刊
作者： Yang, Qiao 1 ; Zhang, Xiaoling 1 ; Huang, Hongliang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst,MOA, Key Lab East China Sea & Ocean Fishery Resources, Sci Observing & Expt Stn Fisheries Resources & En, Beijing, Peoples R China
关键词： NO metabolites;Biological fluids;High-throughput;Microplate
期刊名称：APPLIED MECHANICS AND MATERIALS II, PTS 1 AND 2
ISSN： 1660-9336
年卷期： 2014 年 477-478 卷
页码：
收录情况： SCI
摘要： In this study, we developed a fluorescence measurement's in microplates for high-throughput determination of nitric oxide (NO) metabolites, which is used as a reliable and sensitive marker of NO production, in biological fluids using a pH-independent fluorescence increasing probe, DAMBO-P-H. The sensitivity of this method is lower than that of the other detection methods reported in the literature. This method was demonstrated to be useful for the rapid screening of large numbers of biological and clinical samples, and should be an important tool for further clarifying the physiology, pathology and pharmacology roles of NO.
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