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Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda

文献类型： 外文期刊

作者： Duan, Yafei 1 ; Liu, Ping 1 ; Li, Jitao 1 ; Wang, Yun 3 ; Li, Jian 1 ; Chen, Ping 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab South China Sea Fishery Resources & Explo, Guangzhou 510300, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab South China Sea Fishery Resources & Explo, Guangzho

关键词： Exopalaemon carinicauda;Calreticulin (CRT);Gene cloning;Vibrio anguillarum;White spot syndrome virus (WSSV)

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2014 年 36 卷 1 期

页码：

收录情况： SCI

摘要： Calreticulin (CRT), as a highly conserved endoplasmic reticulum lumina] resident protein, plays important roles in Ca2+ homeostasis, molecular chaperoning and response to viral infection. In this study, a full-length cDNA of CRT (designated EcCRT) was cloned from hemocytes of the ridgetail white prawn Exopalaemorz carinicauda by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of EcCRT was 1725 bp, which contains a 5'-untranslated region (UTR) of 57 bp, 3'-UTR of 453 bp with a poly (A) tail, an open reading frame (ORF) of 1215 bp, encoding a 404 amino-acid polypeptide with the predicted molecular weight of 46.51 kDa and estimated isoelectric point of 4.32. The deduced amino acid sequence of EcCRT shared high identity (82%-85%) with that of other crustaceans. Phylogenetic analysis showed that EcCRT of E. carinicauda was clustered together with CRT of other shrimps, indicating that EcCRT should be a member of the CRT family. Quantitative real-time RT-qPCR analysis indicated that EcCRT was expressed in hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eyestalk, with the highest expression level in hemocytes. After Vibrio anguillarum and WSSV challenge, the expression level of EcCRT transcripts both in the hemocytes and hepatopancreas of E. carinicauda were up-regulated in the first 6 h, respectively. The results suggested that EcCRT might be associated with the immune defenses to V anguillarum and WSSV in E. carinicauda. (C) 2013 Published by Elsevier Ltd.

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[1]The responsive expression of a chitinase gene in the ridgetail white prawn Exopalaemon carinicauda against Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun.

[2]A farnesoic acid O-methyltransferase (FAMeT) from Exopalaemon carinicauda is responsive to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun.

[3]The role of oncoprotein NM23 gene from Exopalaemon carinicauda is response to pathogens challenge and ammonia-N stress. Duan, Yafei,Zhang, Zhe,Li, Jitao,Li, Jian,Ge, Qianqian,Liu, Ping,Li, Jitao,Li, Jian,Ge, Qianqian,Liu, Ping.

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[6]Expression profiles of selenium dependent glutathione peroxidase and glutathione S-transferase from Exopalaemon carinicauda in response to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei.

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[13]A TaqMan real-time PCR assay for survey of white spot syndrome virus (WSSV) infections in Litopenaeus vannamei postlarvae and shrimp of farms in different grow-out seasons. Meng, Xian-Hong,Jang, In Kwon,Seo, Hyung-Chul,Cho, Yeong-Rok,Meng, Xian-Hong.

[14]Immune gene discovery by expressed sequence tag (EST) analysis of hemocytes in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei. 2013

[15]Isolation of Prawn (Exopalaemon carinicauda) Lipopolysaccharide and beta-1, 3-Glucan Binding Protein Gene and Its Expression in Responding to Bacterial and Viral Infections. Ge Qianqian,Li Jitao,Sun Ming,Ge Qianqian,Li Jian,Sun Ming,Zhao Fazhen,Ge Qianqian,Li Jian,Sun Ming,Zhao Fazhen,Duan Yafei. 2016

[16]Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress. Ren Hai,Wu Jianhua,Ren Hai,Li Jian,Li Jitao,Liu Ping,Liang Zhongxiu. 2015

[17]Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress. Ren Hai,Ren Hai,Li Jian,Li Jitao,Ying Yu,Ge Hongxing,Li Dongli,Yu Tianji. 2015

[18]Molecular characterization and expression analysis of the beta-actin gene from the ridgetail white prawn Exopalaemon carinicauda. Liang, J. P.,Nie, G. X.,Liang, J. P.,Ge, Q. Q.,Li, J. T.,Liu, P.,Li, J.,Wang, Y.. 2016

[19]Transcriptome analysis of eyestalk and hemocytes in the ridgetail white prawn Exopalaemon carinicauda: assembly, Annotation and Marker Discovery. Li, Jitao,Li, Jian,Chen, Ping,Liu, Ping,He, Yuying.

[20]cDNA cloning, characterization and expression analysis of ATP-binding cassette transmembrane transporter in Exopalaemon carinicauda. Zhai, Qian-Qian,Li, Jian,Chang, Zhi-Qiang,Zhai, Qian-Qian,Li, Jian,Chang, Zhi-Qiang.

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