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Genotype-environment interactions for survival at low and sub-zero temperatures at varying salinity for channel catfish, hybrid catfish and transgenic channel catfish

文献类型: 外文期刊

作者: Abass, Nermeen Y. 1 ; Elwakil, Houssam E. 2 ; Hemeida, Alaa A. 3 ; Abdelsalam, Nader R. 2 ; Ye, Zhi 1 ; Su, Baofeng 1 ;

作者机构: 1.Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA

2.Univ Alexandria, Dept Agr Bot, Fac Agr Saba Basha, POB 2153, Alexandria, Egypt

3.Menoufia Univ, Inst Genet Engn & Biotechnol, Dept Bioinformat, POB 22857-79, Sadat City, Egypt

4.Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada

5.Chinese Acad Fishery Sci, Minist Agr, Key Lab Freshwater Aquat Biotechnol & Genet Breed, Heilongjiang Fisheries Res Inst, Harbin 150070, Peoples R China

6.King Faisal Univ, Agr & Food Sci Coll,

关键词: Transgenic channel catfish;Salinity;Sub-zero temperature;AFP-ccGH;beta A-GAD65

期刊名称:AQUACULTURE ( 影响因子:4.242; 五年影响因子:4.723 )

ISSN: 0044-8486

年卷期: 2016 年 458 卷

页码:

收录情况: SCI

摘要: Organisms exposed to sub-zero temperatures are at risk of freezing damage. Fingerling channel catfish, Ictalurus punctatus, hybrid catfish (channel catfish female x blue catfish, Ictalurus furcatus, male), channel catfish transgenic for the goldfish glutamate decarboxylase 65 gene driven by the carp beta-actin promoter (beta A-GAD65), and channel catfish transgenic for the catfish growth hormone gene driven by the antifreeze protein promoter (AFP-ccGH) were compared for survival at different temperatures (9.0 degrees C, 6.0 degrees C, 3.0 degrees C, 1.0 degrees C, 0.5 degrees C, 0 degrees C, and -0.5 degrees C) at different salinities (0 ppt, 2.5 ppt, 5 ppt, and 7.5 ppt). The two transgenes were of interest as growth hormone not only affects growth, but also affects osmoregulation, and GAD65 construct could alter gonadotropin with the potential consequence that GnRH affects growth hormone production. Survival was 98-100% for all genetic groups at all salinities between 0 degrees C and 9.0 degrees C. However, large differences were observed at -0.5 degrees C. At 0 ppt salinity, 100% of AFP-ccGH transgenic (T) fingerlings survived, but survival of all other genetic groups was 0-2%. Raising salinity to 2.5 ppt at sub-zero temperature had a strong positive impact on survival as survival rates of AFP-ccGH (T), AFP-ccGH control (C), channel catfish, beta A-GAD65 (T), beta A-GAD65 (C) and hybrid catfish were 100, 100, 98, 76, 100 and 18%, respectively. Increasing salinity further to 5 ppt decreased overall survival, although it was still higher than at 0 ppt. Survival rankings were altered, with means for beta A-GAD65 (T), beta A-GAD65 (C), AFP-ccGH (T), AFP-ccGH(C), channel catfish and hybrid catfish of 69, 0, 15, 22, 0 and 0%, respectively. Mortality was 100% in all genetic groups at -0.5 degrees C and 7.5 ppt demonstrating significant interaction between temperature and salinity. Negative heterosis was observed for the hybrids at low temperature at the respective salinities. (C) 2016 Elsevier B.V. All rights reserved.

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