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The Effect of Muscle-Regulatory Factor Genes and Satellite Cell Response to Recombinant Hsp70 Protein on Megalobrama amblycephala Skeletal Muscle

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文献类型：外文期刊

作者： Zhu, Kecheng ¹ ; Zhang, Dianchang ¹ ; Wang, Huanling ² ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Div Aquaculture & Genet Breeding, Minist Agr,South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Key Lab Freshwater Anim Breeding, Minist Educ,Coll Fishery, Wuhan 430070, Peoples R China

3.Key Lab Fishery Ecol & Environm, Guangzhou, Guangdong, Peoples R China

4.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China

关键词： Hsp70;skeletal muscle;Megalobrama amblycephala;satellite cell

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （影响因子：0.287；五年影响因子：0.593 ）

ISSN： 0792-156X

年卷期： 2016 年 68 卷

页码：

收录情况： SCI

摘要： Hsp70 (70-kDa heat shock protein) plays a key regulatory role in muscle development. In order to evaluate whether recombinant Hsp70 protein (rMaHsp70) of M. amblycephala could affect muscle growth by inducing the expression of Myogenin (MyoG), Myogenic Differentiation Antigen (MyoD), Myogenic factor 5 (Myf5), myogenic regulatory factor 4 (MRF4) and myostatin (MSTN) in muscle, Hsp70 was injected intraperitoneally (IP) at two concentrations: 1.0 mg/mL and 3.4 mg/mL. The mRNA and protein levels of the transcription factor Pax-7 and the protein Hsp70 initially increased, then as concentration levels of injected recombinant M. amblycephala Hsp70 protein rMaHsp70 further increased, the protein Hsp70 decreased. In order to investigate the relationship between muscle hyperplasia, hypertrophy and satellite cell growth following rMaHsp70 administration, the number of satellite cells were analyzed and revealed that the numbers of satellite cells in low concentration group (1 mg/mL) was predominantly higher than in the other concentration groups in both red and white muscle. These results suggest that low concentrations of rMaHsp70 could decrease muscle-related gene expression and increase the number of satellite cells. Our research will be helpful in adapting Hsp70 as a feed additive to enhance M. amblycephala growth.

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