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Transcriptome analysis of Tetrahymena thermophila response to exposure with dihydroartemisinin

文献类型： 外文期刊

作者： Pan, Houjun 1 ; Deng, Meiling 2 ; Zhang, Bin 2 ; Fang, Tiantian 2 ; Liu, Yuguo 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Key Lab Aquat Anim Immune Technol Guangdong Prov,M, Guangzhou 510380, Peoples R China

2.Guangdong Med Univ, Sch Publ Hlth, Dongguan 523808, Peoples R China

关键词： Dihydroartemisinin; Tetrahymena thermophila; Toxic effects; Oxidative stress; Energy metabolism

期刊名称：HELIYON （ 影响因子：4.0； 五年影响因子：4.1 ）

ISSN：

年卷期： 2023 年 9 卷 3 期

页码：

收录情况： SCI

摘要： Dihydroartemisinin (DHA) is a derivative of artemisinin and is toxic to parasites. We used the Tetrahymena thermophila (T. thermophila) as a model to explore DHA toxicity. Results showed that low concentration of DHA (20 mu mol/L) promoted cell proliferation, whereas high concentrations of DHA (40-1280 mu mol/L) inhibited that. Appearance of nucleus was pycnosis by laser scanning confocal microscope. DHA significantly elevated activities of SOD and GSH-Px (P < 0.01) and MDA was markedly increased at high level but decreased at low level (P < 0.01). Further results of transcriptome in T. thermophila treated with different concentration DHA group (0, 20, 160 mu mol/L) showed that differentially expressed genes (DEGs) were involved in oxidation-reduction and metabolism of exogenous substances indicated oxidative stress stimulation. Kyoto Encyclo-pedia of Genes and Genomes showed that DEGs were involved in the cytochrome P450-mediated metabolism of exogenous substances, glutathione metabolism and ABC transport. Remarkably, DNA replication was significantly enriched in low concentration DHA, energy metabolism related pathways and necrotic process were considerably enriched in high concentration DHA. The re-sults of RT-qPCR of 13 DEGs were the same as that of transcriptome, in which the expression of GST and GPx family genes were significantly altered after exposed to high-DHA group. DHA induced oxidative stress damage through disturbing with energy. However, detoxification path-ways in T. thermophila to resist oxidative damage and cell alleviated low concentration DHA stress by regulating antioxidant enzyme. This study provides good practice on pharmacological mechanism of artemisinin-based drugs in antiparasitic.

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