您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of diagnostic markers for a wheat leaf rust resistance gene Lr42 using RNA-sequencing

文献类型： 外文期刊

作者： Liu, Yang 1 ; Chen, Hui 2 ; Li, Chunxin 2 ; Zhang, Lirong 2 ; Shao, Mingqin 2 ; Pang, Yuhui 2 ; Xu, Xiangyang 6 ; Bai, Guihua 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst South Subtrop Crops, Zhanjiang 524013, Guangdong, Peoples R China

2.Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA

3.Henan Acad Agr Sci, Zhengzhou 450002, Henan, Peoples R China

4.Hebei Agr Univ, Coll Plant Protect, Baoding 071000, Hebei, Peoples R China

5.Henan Univ Sci & Technol, Coll Agr, Luoyang 471023, Henan, Peoples R China

6.USDA ARS, Wheat Peanut & Other Field Crop Res Unit, Stillwater, OK 74075 USA

7.USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA

关键词： Aegilops tauschii; Triticum tauschii; Lr42; KASP; Diagnostic marker

期刊名称：CROP JOURNAL （ 影响因子：4.647； 五年影响因子：5.781 ）

ISSN： 2095-5421

年卷期： 2021 年 9 卷 6 期

页码：

收录情况： SCI

摘要： Wheat leaf rust is a prevalent foliar disease in wheat worldwide. Growing resistant cultivars is an effective strategy to minimize the impact of leaf rust on yield and grain quality. Lr42 is a leaf rust resistance gene identified from Aegilops tauschii and is still effective against current predominant leaf rust races in the United States and many other countries. In this study, we developed diagnostic DNA markers for Lr42 using the sequence polymorphisms of a differentially expressed gene (TaRPM1) encoding a putative NBARC protein in the Lr42 candidate region identified by RNA-sequencing of two near-isogenic lines contrasting in Lr42 alleles. Markers were designed based on a deletion mutation and a single nucleotide polymorphism (SNP) in the gene. Haplotype analyses of the newly developed markers in the three diversity panels demonstrated that they are diagnostic for Lr42, and superior to previously used markers in selection accuracy. These markers have the advantages of low cost and easy assay, and they are suitable for marker-assisted selection in breeding programs with either high- or low-throughput marker screening facilities. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台